MRL.Cg-Ccr1tm1Gao Ccr5tm1Blck Faslpr/Cnbc

Status

Available to order

EMMA IDEM:08425
International strain nameMRL.Cg-Ccr1tm1Gao Ccr5tm1Blck Faslpr/Cnbc
Alternative name15L-M
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolCcr5tm1Blck,
Gene/Transgene symbolCcr5

Information from provider

ProviderBruno Luckow
Provider affiliationArbeitsgruppe Klinische Biochemie, Klinikum der Universität München, Medizinische Klinik und Poliklinik IV
Genetic informationOfficial allele symbols: Ccr1tm1Gao Ccr5tm1Blck Faslpr. The chemokine receptors Ccr1 and Ccr5 have been inactivated by homologous recombination and the resulting single knock-outs have been crossed to generate the Ccr1/Ccr5 double knock-out mice. The Ccr1/Ccr5 double-deficient mice have been backcrossed to the autoimmune background MRL/lpr, which carries the spontaneous lpr mutation at the Fas locus.
Phenotypic informationHomozygous:
Mice homozygous for the lymphoproliferation spontaneous mutation (Fas) show systemic autoimmunity, massive lymphadenopathy associated with proliferation of aberrant T cells, arthritis, and immune complex glomerulonephrosis. Autoimmune disease starts at about 3-4 months of age. The simultaneous deletion of the chemokine receptors Ccr1 and Ccr5 does not clearly change severity of the disease.

Heterozygous:
Heterozygous mice at the lpr locus show a much milder form of the autoimmune disease.
Breeding historyThe Ccr1/Ccr5 double-deficient mice were originally on a C57BL/6 background and the mutant allele has been transferred to the MRL/lpr background by backcrossing. Initially for 10 generations, then after several generations of inbreeding homozygous mice, they have been further backcrossed. Currently they are at N13.
ReferencesNone available
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisednot known

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivinghomozygous 0

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • altered response to myocardial infarction / MGI
  • abnormal leukocyte adhesion / MGI
  • abnormal cellular extravasation / MGI
  • decreased mast cell number / MGI
  • decreased susceptibility to type III hypersensitivity reaction / MGI
  • abnormal Kupffer cell morphology / MGI
  • impaired hematopoiesis / MGI
  • abnormal neutrophil physiology / MGI
  • granulomatous inflammation / MGI
  • increased susceptibility to parasitic infection / MGI
  • immune system phenotype / MGI
  • increased susceptibility to fungal infection / MGI
  • increased length of allograft survival / MGI
  • abnormal stria vascularis morphology / MGI
  • increased neutrophil cell number / MGI
  • cortical renal glomerulopathies / MGI
  • enlarged lymph nodes / MGI
  • impaired coordination / MGI
  • abnormal spatial learning / MGI
  • decreased IgG level / MGI
  • conjunctivitis / MGI
  • vasculitis / MGI
  • salivary gland inflammation / MGI
  • premature death / MGI
  • abnormal kidney physiology / MGI
  • abnormal lymph node morphology / MGI
  • increased immunoglobulin level / MGI
  • abnormal immunoglobulin level / MGI
  • increased IgG level / MGI
  • glomerulonephritis / MGI
  • albuminuria / MGI
  • increased urine protein level / MGI
  • dilated renal glomerular capsule / MGI
  • increased autoantibody level / MGI
  • abnormal strial intermediate cell morphology / MGI
  • abnormal conjunctival epithelium morphology / MGI
  • increased anti-double stranded DNA antibody level / MGI
  • increased anti-nuclear antigen antibody level / MGI
  • increased susceptibility to systemic lupus erythematosus / MGI
  • abnormal renal glomerulus morphology / MGI
  • abnormal myocardium layer morphology / MGI
  • vision/eye phenotype / MGI
  • abnormal enzyme/coenzyme activity / MGI
  • CNS inflammation / MGI
  • increased plasma cell number / MGI
  • podocyte foot process effacement / MGI
  • decreased marginal zone B cell number / MGI
  • decreased transitional stage B cell number / MGI
  • decreased mature B cell number / MGI
  • decreased immature B cell number / MGI
  • decreased spleen germinal center number / MGI
  • renal glomerulus hypertrophy / MGI
  • expanded mesangial matrix / MGI
  • mesangial cell hyperplasia / MGI
  • glomerular crescent / MGI
  • increased or absent threshold for auditory brainstem response / MGI
  • abnormal thyroid gland morphology / MGI
  • enlarged spleen / MGI
  • enlarged thymus / MGI
  • skin lesions / MGI
  • abnormal cardiovascular system physiology / MGI
  • abnormal immune system physiology / MGI
  • vascular inflammation / MGI
  • abnormal gland morphology / MGI
  • abnormal thymus medulla morphology / MGI
  • abnormal T cell physiology / MGI
  • increased IgM level / MGI
  • increased IgA level / MGI
  • abnormal type III hypersensitivity reaction / MGI
  • abnormal immune system organ morphology / MGI
  • joint swelling / MGI
  • increased circulating serum albumin level / MGI
  • thyroid inflammation / MGI
  • thymus atrophy / MGI
  • abnormal submandibular gland morphology / MGI
  • abnormal coronary artery morphology / MGI
  • abnormal thyroid follicle morphology / MGI
  • increased anti-single stranded DNA antibody level / MGI
  • increased anti-erythrocyte antigen antibody level / MGI
  • abnormal B cell morphology / MGI
  • increased thymus weight / MGI
  • abnormal cytotoxic T cell physiology / MGI
  • abnormal T cell proliferation / MGI
  • increased susceptibility to autoimmune disorder / MGI
  • endocrine/exocrine gland phenotype / MGI
  • abnormal circulating protein level / MGI
  • abnormal T-helper 2 physiology / MGI
  • increased circulating total protein level / MGI
  • abnormal T cell morphology / MGI
  • lymph node hyperplasia / MGI
  • enlarged Peyer's patches / MGI
  • abnormal marginal zone B cell morphology / MGI
  • increased IgG1 level / MGI
  • increased IgG2a level / MGI
  • increased IgG2b level / MGI
  • decreased interleukin-2 secretion / MGI
  • abnormal interleukin level / MGI
  • abnormal B cell receptor editing / MGI
  • abnormal lymph node cell ratio / MGI
  • submandibular gland inflammation / MGI
  • lacrimal gland inflammation / MGI
  • abnormal glomerular capillary morphology / MGI
  • renal cast / MGI
  • increased renal glomerulus basement membrane thickness / MGI
  • increased kidney cell proliferation / MGI
  • abnormal glomerular capillary endothelium morphology / MGI
  • thymus cortex atrophy / MGI
  • abnormal hepatocyte morphology / MGI
  • abnormal liver physiology / MGI
  • demyelination / MGI
  • focal hepatic necrosis / MGI
  • brain inflammation / MGI
  • abnormal kidney morphology / MGI
  • increased susceptibility to bacterial infection / MGI
  • increased susceptibility to viral infection / MGI
  • increased anti-histone antibody level / MGI
  • increased follicular B cell number / MGI
  • increased marginal zone B cell number / MGI
  • increased transitional stage B cell number / MGI
  • increased immature B cell number / MGI
  • decreased B cell apoptosis / MGI
  • abnormal splenic cell ratio / MGI
  • decreased hepatocyte apoptosis / MGI
  • increased splenocyte number / MGI
  • abnormal NK cell physiology / MGI
  • abnormal renal glomerulus basement membrane morphology / MGI
  • increased renal glomerulus apoptosis / MGI
  • increased spleen weight / MGI
  • increased blood urea nitrogen level / MGI
  • abnormal airway responsiveness / MGI
  • decreased eosinophil cell number / MGI
  • increased apoptosis / MGI
  • abnormal B cell activation / MGI
  • mortality/aging / MGI
  • decreased activated T cell number / MGI
  • autoimmune response / MGI
  • decreased B cell number / MGI
  • increased double-negative T cell number / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • abnormal leukocyte morphology / MGI
  • impaired leukocyte tethering or rolling / MGI
  • ruffled hair / MGI
  • increased body weight / MGI
  • increased tumor incidence / MGI
  • decreased susceptibility to autoimmune diabetes / MGI
  • abnormal skin condition / MGI
  • epidermal necrosis / MGI
  • kidney failure / MGI
  • decreased neuron apoptosis / MGI
  • decreased apoptosis / MGI
  • abnormal vagina weight / MGI
  • lacrimal gland atrophy / MGI
  • cardiovascular system phenotype / MGI
  • abnormal stria vascularis vasculature morphology / MGI
  • increased susceptibility to type III hypersensitivity reaction / MGI
  • increased sensitivity to induced morbidity/mortality / MGI
  • decreased macrophage apoptosis / MGI
  • increased IgG3 level / MGI
  • kidney inflammation / MGI
  • increased gamma-delta T cell number / MGI
  • abnormal immune system morphology / MGI

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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