NOD.129P2(B6)-Nos2tm1Lau/Ieg

Status

Available to order

EMMA IDEM:09914
International strain nameNOD.129P2(B6)-Nos2tm1Lau/Ieg
Alternative nameNOD.B6;129P2-Nos2>tm1Lau
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolNos2tm1Lau,
Gene/Transgene symbolNos2

Information from provider

ProviderVolker Burkart
Provider affiliationInstitut für Klinische Diabetologie, Deutsches-Diabetes-Zentrum
Genetic informationReplacement of exons 12 and 13 encoding the calmodulin-binding domain of the iNOS (Nos2) gene by a neomycin cassette.
Phenotypic informationHomozygous:
After backcross on the NOD background no homozygous offspring was obtained anymore.

Heterozygous:
Heterozygous mice are viable and fertile and show no apparent abnormalities. No further characterization was performed.
Breeding historyB6;129P2-Nos2tm1Lau mice, provided by Paula Sherman, were backcrossed for 32 generations on the NOD/Lt background. For the last 9 generations intercross of heterozygous mice was performed.
References
  • Mice lacking inducible nitric oxide synthase are not resistant to lipopolysaccharide-induced death.;Laubach V E, Shesely E G, Smithies O, Sherman P A, ;1995;Proceedings of the National Academy of Sciences of the United States of America;92;10688-92; 7479866
Homozygous fertileno
Homozygous viableno
Homozygous matings requiredno
Immunocompromisednot known

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivingheterozygous NOD

Disease and phenotype information

MGI phenotypes (allele matching)
  • abnormal sleep pattern / MGI
  • abnormal inflammatory response / MGI
  • decreased inflammatory response / MGI
  • abnormal surfactant physiology / MGI
  • abnormal brain wave pattern / MGI
  • abnormal circadian sleep/wake cycle / MGI
  • decreased circulating alanine transaminase level / MGI
  • decreased susceptibility to injury / MGI
  • decreased circulating aspartate transaminase level / MGI
  • immune system phenotype / MGI
  • abnormal systemic arterial blood pressure / MGI
  • abnormal immune system morphology / MGI
  • abnormal testis morphology / MGI
  • decreased white adipose tissue amount / MGI
  • abnormal gametogenesis / MGI
  • abnormal reproductive system morphology / MGI
  • altered tumor susceptibility / MGI
  • increased susceptibility to bacterial infection / MGI
  • abnormal adaptive immunity / MGI
  • abnormal sperm number / MGI
  • increased growth rate / MGI
  • increased insulin sensitivity / MGI
  • increased testis weight / MGI
  • skin inflammation / MGI
  • abnormal male meiosis / MGI
  • improved glucose tolerance / MGI
  • abnormal enzyme/coenzyme level / MGI
  • increased T cell proliferation / MGI
  • homeostasis/metabolism phenotype / MGI
  • cardiovascular system phenotype / MGI
  • abnormal fertilization / MGI
  • increased circulating creatinine level / MGI
  • increased circulating antidiuretic hormone level / MGI
  • decreased circulating leptin level / MGI
  • enlarged seminiferous tubules / MGI
  • increased single-positive T cell number / MGI
  • enlarged inguinal lymph nodes / MGI
  • abnormal blood homeostasis / MGI
  • increased sensitivity to induced morbidity/mortality / MGI
  • increased food intake / MGI
  • increased urine pH / MGI
  • abnormal heart morphology / MGI
  • abnormal corpus callosum morphology / MGI
  • abnormal eating behavior / MGI
  • abnormal glucose homeostasis / MGI
  • abnormal respiratory system physiology / MGI
  • abnormal blood coagulation / MGI
  • enhanced wound healing / MGI
  • abnormal bone mineralization / MGI
  • increased susceptibility to pharmacologically induced seizures / MGI
  • abnormal frequency of paradoxical sleep / MGI
  • decreased susceptibility to induced choroidal neovascularization / MGI
  • abnormal physiological neovascularization / MGI
  • abnormal bone structure / MGI
  • increased left ventricle developed pressure / MGI
  • increased mortality induced by ionizing radiation / MGI
  • abnormal respiratory bronchiole morphology / MGI
  • abnormal osteoclast morphology / MGI
  • abnormal heart ventricle morphology / MGI
  • abnormal myocardium layer morphology / MGI
  • respiratory system phenotype / MGI
  • abnormal platelet physiology / MGI
  • decreased systemic arterial systolic blood pressure / MGI
  • decreased susceptibility to induced colitis / MGI
  • decreased oligodendrocyte number / MGI
  • mortality/aging / MGI
  • improved central nervous system regeneration / MGI

Literature references

  • Mice lacking inducible nitric oxide synthase are not resistant to lipopolysaccharide-induced death.;Laubach V E, Shesely E G, Smithies O, Sherman P A, ;1995;Proceedings of the National Academy of Sciences of the United States of America;92;10688-92; 7479866

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.


Search
INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).