B6N.Cg-Stat1^tm1Dlv Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} Col1a1^{tm1(tetO-Stat1)Biat}/Biat

Status	Available to order
EMMA ID	EM:09938
International strain name	B6N.Cg-Stat1^tm1Dlv Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} Col1a1^{tm1(tetO-Stat1)Biat}/Biat
Alternative name	C57BL/6N-Stat1, Gt(ROSA)26Sor, Col1a1
Strain type	Targeted Mutant Strains : Conditional mutation
Allele/Transgene symbol	Col1a1^{tm1(tetO-Stat1)Biat},
Gene/Transgene symbol	Col1a1

Information from provider

Provider	Mathias Müller
Provider affiliation	Department for Biomedical Sciences, University of Veterinary Medicine Vienna (Vetmeduni Vienna)
Additional owner	Prof. Thomas Rülicke, University of Veterinary Medicine, Institute of Laboratory Animal Science, Vienna, Austria
Genetic information	Stat1^tm1Dlv: A neomycin resistance cassette replaced 5.7 kb of sequence, including 3 exons and a portion of a fourth encoding amino acids 221-365. This allele produces a partial nonfunctional protein product. (J:31325) Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae}: An optimized form of reverse tetracycline controlled transactivator (rtTA-M2) was inserted downstream of the Gt(ROSA)26Sor promoter and was followed by a PGK-puro selection cassette. This mutant form of rtTA termed M2 has five amino acid substitutions in the tetR moiety of tTA: S12G, E19G, A56P, D148E and H179R. This mutated form of transactivatory protein has increased doxycycline sensitivity. Mice have widespread expression of the rtTA-M2 protein. (J:98920) Col1a1^{tm1(tetO-Stat1)Biat}: RMCE on KH2 ES cells introduced into the 3' UTR a tetracycline responsive element driving inducible expression of the FLAG-tagged mouse cDNA. (J:212694)
Phenotypic information	Homozygous: n/a Heterozygous: immunological phenotype - see paper
Breeding history	The mice were backcrossed to the C57BL/6N background by speed congenics (Teppner et al. 2004; Lab Anim. 38:406–412. 10.1258/0023677041958882).
References	Inducible, dose-adjustable and time-restricted reconstitution of STAT1 deficiency in vivo.;Leitner Nicole R, Lassnig Caroline, Rom Rita, Heider Susanne, Bago-Horvath Zsuzsanna, Eferl Robert, Müller Simone, Kolbe Thomas, Kenner Lukas, Rülicke Thomas, Strobl Birgit, Müller Mathias, ;2014;PloS one;9;e86608; 24489749
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	not known

Information from EMMA

Archiving centre	University of Veterinary Medicine, Vienna, Austria
Animals used for archiving	homozygous C57BL/6N
Breeding at archiving centre	Breeding of animals for homozygous embryo production

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Ehlers-Danlos/osteogenesis imperfecta syndrome / Orphanet_230857
Osteogenesis imperfecta type 3 / Orphanet_216812
Arthrochalasia Ehlers-Danlos syndrome / Orphanet_1899
Classical Ehlers-Danlos syndrome / Orphanet_287
Osteogenesis imperfecta type 2 / Orphanet_216804
Osteogenesis imperfecta type 4 / Orphanet_216820
Caffey disease / Orphanet_1310
High bone mass osteogenesis imperfecta / Orphanet_314029
Osteogenesis imperfecta type 1 / Orphanet_216796
Autoimmune enteropathy and endocrinopathy-susceptibility to chronic infections syndrome / Orphanet_391487
Susceptibility to viral and mycobacterial infections due to STAT1 deficiency / Orphanet_391311
Mendelian susceptibility to mycobacterial diseases due to partial STAT1 deficiency / Orphanet_319595

MGI phenotypes (allele matching)

increased mammary adenocarcinoma incidence / MGI
impaired natural killer cell mediated cytotoxicity / MGI
increased circulating interferon-gamma level / MGI
increased circulating interleukin-12b level / MGI
abnormal NK cell physiology / MGI
decreased level of surface class I molecules / MGI
increased susceptibility to bacterial infection / MGI
increased susceptibility to viral infection / MGI
abnormal MHC II cell surface expression on macrophages / MGI
vision/eye phenotype / MGI
decreased susceptibility to endotoxin shock / MGI
abnormal intestine morphology / MGI
decreased body size / MGI
multifocal hepatic necrosis / MGI
abnormal leukocyte physiology / MGI
lethality at weaning, complete penetrance / MGI

Literature references

Inducible, dose-adjustable and time-restricted reconstitution of STAT1 deficiency in vivo.;Leitner Nicole R, Lassnig Caroline, Rom Rita, Heider Susanne, Bago-Horvath Zsuzsanna, Eferl Robert, Müller Simone, Kolbe Thomas, Kenner Lukas, Rülicke Thomas, Strobl Birgit, Müller Mathias, ;2014;PloS one;9;e86608; 24489749

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6N.Cg-Stat1tm1Dlv Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm1(tetO-Stat1)Biat/Biat