The recent emergence of gene editing techniques, such as the CRISPR-Cas9 system, has allowed researchers to quickly produce very specific mouse mutants on highly defined genetic backgrounds. Previous to now, that would have been expensive both financially and in terms of time.
There are many approaches that can be used to generate CRISPR-Cas9 edited mouse models including the use of electroporation. This course will teach the use CRISPR-CAS9 and electroporation to generate on-target founder mice for both deletions and point mutations.
This technique is relatively simple to learn when compared with the time required to become competent with pronuclear injections.