The personalised cancer mouse model generation service involves project design including prediction of off-target sites, preparation of sgRNA’s and Cas9 mRNA/protein, and injection into zygotes to generate F0 founder mutant animals (C57BL/6N or C57BL/6J genetic background preferred). Selected F0 animals will be bred to germline to produce F1 genome edited animals. Possible allele types that can be generated are indels, exon deletions (< 10kb) and point mutation insertions. Newly developed mouse models will be made available to selected applicants within an average of 12 months following provision of all required information to start the mouse production.
INFRAFRONTIER Cancer Services
Over the past 60 years, mouse models have become indispensable in the study of carcinogenesis. Their major advantage lies in the ease with which they can be genetically engineered. The access to conditional lines that allow temporal and spatial activation of oncogenesis has helped researchers to understand basic concepts of cancer, as well as develop and test promising treatments (Hirst and Balmain 2004).