INFRAFRONTIER Partners

BSRC Alexander Fleming, Vari-Athens, Greece

Fleming Logo

Biomedical Sciences Research Center "Alexander Fleming"
34 Fleming Street
16672, Vari-Athens
Greece

Fleming building

Fleming is a non-profit research organization that operates under the supervision of the General Secretariat for Research and Technology of the Hellenic Ministry of Development. The center was established in 1998 and today is actively involved in cutting edge research in biomedical sciences. Fleming is housed in a 6.000m2 building located in Vari near Athens, and currently hosts 14 research groups distributed in four divisions (Immunology, Molecular Oncology, Molecular Biology & Genetics and Cellular & Developmental Biology). Fleming's research activities focus in the areas of disease pathway and target identification, inter- and intra-cellular signalling, post-transcriptional regulation of gene expression, functional genomics and preclinical drug development. Integral to Fleming's research activities are several platforms of genetic permutation in the mouse and their application in human disease modelling. These systems have served as a basis for multiple academic collaborations in R&D under FP6 and FP7 European programs focusing on the mouse as a research tool, as well as for interactions with the international biotech and pharmaceutical industry. Fleming's transgenic facility is a core facility for the generation and cryopreservation of genetically modified mice and since 2009 comprises the Greek EMMA node. The facility is complemented by Fleming's animal house (ISO 9001) covering an area of approximately 600m2  that has a housing capacity of more than 18.000 mice and is equipped with highly automated systems that provide optimal conditions for the reproduction and maintenance of inbred strains or genetically engineered mice. Fleming also operates a number of other top-level facility units including expression profiling, genotyping, flow cytometry, histopathology, and proteomics units offering state-of-the-art services to researchers and companies from Greece and abroad. The above infrastructures are currently being upgraded and expanded through INFRAFRONTIER-GR, the Greek node of INFRAFRONTIER, to which the Hellenic Ministry of Development has recently committed 3,9 million Euro. INFRAFRONTIER-GR is a consortium of three top research centers in Greece, coordinated by Fleming, and is currently in implementation phase. Fleming participates in several EC FP6 and FP7 projects using the mouse as a research tool including MUGEN, EUMODIC, CASIMIR, I-DCC, CREATE, MASTERSWITCH, INFLACARE, IMI BTCURE and others.

 

Key personnel

Node Director/PI: Dr. George Kollias, Node Managing Director: Dr. Dimitris L. Kontoyiannis

Mouse resource enquiries: transgenics@fleming.gr

Selected references

  • Koliaraki V, Roulis M, Kollias G. 2012. "Tpl2 regulates intestinal myofibroblast HGF release to suppress colitis-associated tumorigenesis." J Clin Invest. 122:4231-4242. PMID: 23064365
  • Nikolaou K, Tsagaratou A, Eftychi C, Kollias G, Mosialos G, Talianidis I . 2012. "Inactivation of the deubiquitinase CYLD in hepatocytes causes apoptosis, inflammation, fibrosis and cancer." Cancer Cell . 21:738-750 PMID: 22698400
  • Yiakouvaki A, Dimitriou M, Karakasiliotis I, Eftychi C, Theocharis S, Kontoyiannis DL. 2012. "Myeloid cell expression of the RNA-binding protein HuR protects mice from pathologic inflammation and colorectal carcinogenesis." J Clin Invest. 122:48-61 PMID: 22201685
CERBM-GIE, Institut Clinique de la Souris, Strasbourg, France

 ICS Logo

Institut Clinique de La Souris
1, rue Laurent Fries

BP 10142
67404 Illkirch Cedex
France

ICS building

The Institut Clinique de la Souris is a national infrastructure devoted to mouse genomics and operated by the CERBM-GIE (directed by Prof. Brigitte Kieffer), a non-profit biomedical research organisation funded in part and controlled by an alliance of French research agencies (CNRS and INSERM) and by the University of Strasbourg. Located in Illkirch near Strasbourg, the ICS is part of the National INFRASTRUCTURE for Biology and Health PHENOMIN, laureate of the Investment for the future call in 2011.

The CERBM-GIE has a long tradition of collaboration with both the academic community and pharmaceutical industries, in particular in the fields of mouse genetics, with an international recognition illustrated by the major European programs to which ICS participates (EUCOMM, EUMORPHIA, EUMODIC, EMMA and INFRAFRONTIER). The CERBM-GIE has a strong expertise in participating to FP6 and FP7 projects, notably in mouse functional genomics, and is also involved with the Strasbourg University in academic training. ICS is equipped with integrated facilities for genetics, cellular and molecular biology, and notably for creation and characterization of genetically engineered mouse models and their behaviour and cognitive assessments. With the new project PHENOMIN (part of the call for projects called National Infrastructures in Biology and Health), the goal of the ICS is to give birth to a national infrastructure specialized in development, analysis and conservation of mice models. This project federates teams of excellence in mouse genetics (ICS, CNRS-TAAM and INSERM-CIPHE).

Key personnel

Dr. Yann Herault (ICS/MCI, ICS director) has been involved in about 17 EU-funded projects since 2001, to tackle human diseases using mouse genetics, and notably trisomy 21, a leading cause of cognitive disabilities. He is involved in translational research and the use of mouse models of human disease. He is coordinating PHENOMIN the national infrastructure for mouse phenogenomics and the large scale infrastructure CELPHEDIA for animal models. Dr. Tania Sorg  is the Deputy Director of the ICS. She is leading the ICS department for phenotyping, which analysed more than 150 mouse mutant lines per year for the scientific community. Dr. Abdel Ayadi, Mouse Facility Manager of the Institut Clinique de la Souris (ICS). Between 2000 and 2007 he worked as project manager in the field of mouse transgenesis in both industry and academia (ICS) and  since 2008 is in charge of the ICS Mouse supporting service department for ICS operations. Dr. Abdel Ayadi  is a member of the EMMA as Archive Manager. Dr. Mohammed Selloum is the European program manager at the ICS and contributed to several successful European funded projects with other European mouse clinics (EUMORPHIA and EUMODIC and INFRAFRONTIER). Dr. Hamid Meziane is the specialist of the behavioural and cognitive analysis of mouse models at the ICS. Dr. Olivia Wendling is the co-head of the Histopathology and Embryology service. Dr. Marie-France Champy is the specialist for metabolism and clinical biochemistry of mouse mutants. Philippe André is in charge of the cryopreservation and rederivation service.

Selected references

  • Birling MC, Dierich A, Jacquot S, Hérault Y, Pavlovic G. 2012. Highly-efficient, fluorescent, locus directed cre and FlpO deleter mice on a pure C57BL/6N genetic background. Genesis. 50:482-489. PMID: 22121025
  • Ayadi A, Birling MC, Bottomley J, Bussell J, Fuchs H, Fray M, Gailus-Durner V, Greenaway S, Houghton R, Karp N, Leblanc S, Lengger C, Maier H, Mallon AM, Marschall S, Melvin D, Morgan H, Pavlovic G, Ryder E, Skarnes WC, Selloum M, Ramirez-Solis R, Sorg T, Teboul L, Vasseur L, Walling A, Weaver T, Wells S, White JK, Bradley A, Adams DJ, Steel KP, Hrabé de Angelis M, Brown SD, Herault Y. 2012. Mouse large-scale phenotyping initiatives: overview of the European Mouse Disease Clinic (EUMODIC) and of the Wellcome Trust Sanger Institute Mouse Genetics Project. Mamm Genome 9: 600-610. PMID: 22961258
  • Danglot L, Zylbersztejn K, Petkovic M, Gauberti M, Meziane H, Combe R, Champy MF, Birling MC, Pavlovic G, Bizot JC, Trovero F, Della Ragione F, Proux-Gillardeaux V, Sorg T, Vivien D, D'Esposito M, Galli T. 2012. Absence of TI-VAMP/Vamp7 leads to increased anxiety in mice.  J Neurosci 8: 1962-1968. PMID: 22323709
  • Gofflot F, Wendling O, Chartoire N, Birling MC, Warot X, Auwerx J. 2011. Characterization and Validation of Cre-Driver Mouse Lines. Curr. Protoc. Mouse Biol.1:1-15
  • Champy MF, Le Voci L, Selloum M, Peterson LB, Cumiskey AM, Blom D. 2011. Reduced body weight in male Tspan8-deficient mice. Int J Obes. 35:605-17
CIPHE-INSERM, Marseille, France

CIPHE logo

Centre d'Immunophénomique - CIPHE
163 avenue de Luminy - Case 936
13288 Marseille Cedex 09
France

 CIPHE Building

 The Centre for ImmunoPHEnomics (CIPHE) is a newly opened 3500 m2 state-of-the-art facility that develops and analyzes in a massively parallel and standardized mode knock-in and knock-out mouse models to understand the function of the immune system under normal and infectious conditions. CIPHE, with its cutting-edge expertise in mouse genetics and immunology, is an open access platform dedicated to create innovative customized mouse models and pioneer systemic immunophenotyping of mutant mice confronted to infections. CIPHE comprises a knockin-knockout booster unit, a microinjection and cryopreservation unit, an immunophenotyping unit, a BSL3 facility capable of live imaging of infectious processes, and a EOPS animal facility. These advanced core facilities are instrumental in deciphering at the systemic level the complexity of the immune system. Through a series of almost 300 quantitative parameters, CIPHE has the unique ability to phenotype all the cellular components of the innate and adaptive immune system under normal conditions or when confronted to pathogens. It integrates current cutting-edge knowledge in flow and mass cytometry and advanced microscopy. CIPHE is in the process of building long-term collaboration with leading research institutes worldwide and will reinforce cooperation with industrial pharmaceutical partners. This will ensure excellence in collaborative training activities including short courses aimed to provide theoretical knowledge and practical training in the field of mouse genetics, immunophenotyping and bio-imaging in confined conditions.

CIPHE is involved as a "technological core" in several large-scale European projects (MUGEN, MASTERSWITCH and SYBILLA). CIPHE is one of the founding members of PHENOMIN, the French infrastructure for phenogenomics together with Institut Clinique de la Souris (Strasbourg) and TAAM (Orléans). CIPHE belongs to the INFRAFRONTIER and International Mouse Phenotyping Consortium (IMPC). Therefore, CIPHE is a research instrument that copes with the immunophenotyping of the hundreds of mouse mutants that are in the process of being generated in the frame of IMPC. Moreover to reinforce this synergy, CIPHE participates in the Immgen2 project. At the regional level, CIPHE benefits from its close collaboration with the Centre d'Immunologie de Marseille-Luminy (CIML). Moreover CIPHE has tight collaborations with academic laboratories with an interest for development biology (Institut de Biologie du Développement de Marseille Luminy - IBDML), oncology (Centre de Recherche sur le Cancer de Marseille - CRCM) and myology (Hôpital de la Timone, Marseille).

Key personnel

Dr. Bernard Malissen is  the Director of CIPHE, CNRS Investigator, honorary member of the American Association of Immunologists and  member of the French Academy of Sciences. Dr. Marie Malissen, head of the Immunophenotyping module and of the Archiving / Import-Export / Coordination IMPC module of CIPHE, CNRS Investigator and team leader at Centre d'Immunologie de Marseille-Luminy (CIML). Dr. Malissen has a long-standing contribution in the fields of mouse genetics and immunology. In the frame of CIPHE activities, Marie Malissen will manage exhaustive phenotyping of mutant mice and immune responses in standard, inflammation and infection conditions by using multiparameter flow and mass cytometry. Moreover, she will coordinate the services of cryopreservation of mouse lines produced at CIPHE and activities of IMPC implemented within different technological units. Dr Jean-Pierre GORVEL, Head of the BSL3 module of CIPHE and CNRS Investigator. Jean-Pierre Gorvel has a long record of contributions in the field of cellular microbiology. He is an expert on intracellular bacteria and will study in the frame of CIPHE the immune responses of mutant mice challenged with Salmonella Thyphimurium, Brucella spp including melitensis and abortus, Mycobacterium spp including tuberculosis and avium, Staphylococcus aureus and Coxiella burnettii. Jean-Pierre Gorvel has also expertise in approaches aiming at imaging of infectious processes in vivo.

Selected references

  • Barquero-Calvo, E., Martirosyan, A., Ordonez-Rueda, D., Arce-Gorvel, V., Alfaro-Alarcon, A., Lepidi, H., Malissen, B., Malissen, M., Gorvel, J.P., and Moreno, E. (2013). Neutrophils Exert a Suppressive Effect on Th1 Responses to Intracellular Pathogen Brucella abortus. PLoS Pathog 9, e1003167. PMID: 23458832
  • Ordonez-Rueda, D., Jonsson, F., Mancardi, D.A., Zhao, W., Malzac, A., Liang, Y., Bertosio, E., Grenot, P., Blanquet, V., Sabrautzki, S., et al. (2012). A hypomorphic mutation in the Gfi1 transcriptional repressor results in a novel form of neutropenia. Eur J Immunol 42, 2395-2408.
    PMID: 22684987
  • Henri, S., Poulin, L.F., Tamoutounour, S., Ardouin, L., Guilliams, M., de Bovis, B., Devilard, E., Viret, C., Azukizawa, H., Kissenpfennig, A., and Malissen, B. (2010). CD207+ CD103+ dermal dendritic cells cross-present keratinocyte-derived antigens irrespective of the presence of Langerhans cells. J Exp Med 207, 189-206
    PMID: 20038600.
CNR, Monterotondo, Italy

CNR logo

Consiglio Nazionale delle Ricerche, Monterotondo
A. Buzzati-Traverso" International Campus I-00015
Monterotondo Scalo (Roma) Italy

CNR campus

CNR is the largest public Italian institution for multidisciplinary scientific research and technological development. The CNR’s unit “EMMA-Monterotondo Campus International Development” manages the European Mouse Mutant Archive (EMMA) core structure and the Mouse Clinic, at the "A. Buzzati-Traverso" International Campus in Monterotondo (Roma). The Mouse Clinic is establishing new primary phenotyping infrastructures, as a part of the International Mouse Phenotyping Consortium (IMPC). The EMMA Monterotondo facilities were establishment in 1996, by CNR and its international partners, and include SPF barrier and quarantine areas and fully-equipped laboratories for mutant production, cryo-preservation, genotyping and sanitary analysis, in the context of the reference international consortia (IMSR, IKMC, etc.). The EMMA unit at Monterotondo also manages the curation of strain data resources. With an estimated total capacity of >50,000 live animals and >100,000 frozen embryo/gamete vials, the EMMA Monterotondo repository for mouse research is the largest in Italy and the only national archive with in-house production, processing and quality control of banked strains. The EMMA core structure and the Mouse Clinic at the Monterotondo Campus are components of the INFRAFRONTIER Preparatory Phase project (EU-ESFRI Roadmap and Italian Roadmap for large-scale research infrastructures).

Key personnel 

Glauco Tocchini-Valentini is the Head of the CNR’s unit “EMMA-Monterotondo Campus International Development” and a founder of the EMMA network. He has been the administrative co-ordinator of the EU FP EMMA grants. He has been/is a partner of the FP5-FP7 EUMORPHIA, EUMODIC, MUGEN, EURASNET, EUCOMM, EUCOMMtools, PHENOSCALE projects for mouse functional genomics, of IMSR, IKMC, IMPC consortia, of the INFRAFRONTIER Preparatory Phase and PRIME, CASIMIR, InfraCOMP Coordination Actions. The CNR Monterotondo staff members have participated in the EMMA network since its establishment in 1996.

Mouse resource enquiries: mouse.resources@emma.cnr.it

Selected references

  • Avatar pre-tRNAs help elucidate the properties of tRNA-splicing endonucleases that produce tRNA from permuted genes. Tocchini-Valentini GD, Tocchini-Valentini GP. Proc Natl Acad Sci U S A. 2012 Dec 26;109(52):21325-9. doi: 10.1073/pnas.1219336110. PMID: 23236183
  • Yeast pheromone receptor genes STE2 and STE3 are differently regulated at the transcription and polyadenylation level. Di Segni G, Gastaldi S, Zamboni M, Tocchini-Valentini GP. Proc Natl Acad Sci U S A. 2011 Oct 11;108(41):17082-6. doi: 10.1073/pnas.1114648108. PMID: 21969566
  • Evolution of introns in the archaeal world. Tocchini-Valentini GD, Fruscoloni P, Tocchini-Valentini GP. Proc Natl Acad Sci U S A. 2011 Mar 22;108(12):4782-7. doi: 10.1073/pnas.1100862108. PMID: 21383132
CNRS, France

CNRS logo

Institut de Transgenose
3B rue de la Ferollerie
45071 ORLEANS Cedex 2
France

CNRS-TAAM building

The Transgenesis, Archiving and Distribution of Animal Model (TAAM) is a core facility operated by the National Center for Scientific Research (CNRS) in France and part of the National Infrastructure for Biology and Health PHENOMIN. The TAAM holds three departments: (1) the SEAT for transgenesis in Villejuif (Head: Karelia Ruffert-Lipson); (2) the CDTA for zootechny, archiving and distribution in Orleans (Head: P Lopes Pereira); and (3) the CIPA for small animal imaging (Head: S Lerondel). The TAAM has been a founding member of the European Mouse Mutant Archive (EMMA) since 1996 and is a well-known national service unit devoted to mouse genetics since 2001 inside the French network for Infrastructure in life science and agronomy. TAAM is a member of the national network CELPHEDIA whose objective is to develop innovative technologies to provide animal models for use in fundamental and clinical research. The TAAM participates in several EU projects in FP4 (EMMA), in FP5 (EMMAnet, EMMAwork, Eurocomp, Eumorphia, OxPrions and T21-TARGETS) and in FP6 (EMMAinf, EUMODIC, Aneuploidy, TB-React, MPCM) and is a founding member of the Federation of International Mouse Resources (FIMRe). Since 2008, the TAAM is certified for quality management system following the ISO 9001/2000 guidelines. The TAAM has contributed to archiving and distribution of mutant mice under all the EMMA funded projects with a high level profile for distribution inside the network. The infrastructure consists of several devoted mouse facilities which currently support the housing of 12000 cages (8000 breeding / 4000 experimentation). A 200 m2 quarantine area, which is completely separated from the main breeding facility. All animals obtained from outside can be settled and bred, if necessary, in this quarantine area before embryo transfer and/or cryopreservation. For immunodeficient mice, specific housing is provided in IVC racks or isolators. Five SPF facilities,1 Bioconfinment Level 2 area for gene therapy and infectious disease research, 40 isolators (30 cages) are devoted to mouse lines under highest health conditions (SOPF, axenic, gnotoxenic). Each year, the TAAM rederives more than 120 mouse strains and cryopreserves about 100 lines. Moreover the facility provides health monitoring, genetic monitoring and breeding services to more than 450 internal and external users. Since 2006, 1200 strains have been archived and more than 80 000 mice were distributed to laboratories in France and  around the world.

Key personnel

Dr. Cécile Fremond (Veterinarian, TAAM Director since january 2014 succeeding to Dr Yann Herault) is Doctor in veterinary medicine. She integrated the French National Center for Scientific Research (CNRS) in 1999 as the veterinarian of the TAAM. She prepared in parallel a PhD  in the domain of innate immunology namely to understand the role of implicated receptors and signalling pathways involved in the immune response to Mycobacterium tuberculosis. She is involved in PHENOMIN the national infrastructure for mouse phenogenomics and the large scale infrastructure CELPHEDIA for animal models. Dr. Karelia Ruffert-Lipson (Department head of transgenesis) has used transgenic mouse models during her professional career. Her background is in neuroscience/immunology, she has generated transgenic mouse lines using several techniques and has used a wide range of methods in animal experimentation. Dr. Patricia Lopes Pereira (Department head of zootechny) is research engineer. She has an expertise in using mouse for research animal and has participated in the development of experimental procedures using mouse as a model system. She works with 11 services: 6 animal house technical managers, and additional services for genetic control, health monitoring, cryopreservation, decontamination and rederivation. Dr. Stéphanie Lerondel (Department head for imaging) began her formation in biology as agronomy engineer, prepared a PhD thesis on research dealing about development of in vivo imaging strategies. She completed her cursus by a Prof MS degree in Quality Assurance with specialization in QA of imaging dedicated to biopharmaceutical research, then a post-doc in an international CRO in pharmacotoxicology. In 2002, she was appointed by the french National Center for Scientific Research (CNRS) to be in charge of the Department of Imaging (CIPA) for functional explorations and phenotyping of transgenic mice.

Mouse resource enquiries: twgorleans@cnrs-orleans.fr

Selected references

  • Le Bourhis L, Martin E, Péguillet I, Guihot A, Froux N, Coré M, Lévy E, Dusseaux M, Meyssonnier V, Premel V, Ngo C, Riteau B, Duban L, Robert D, Huang S, Rottman M, Soudais C, Lantz O. 2010. Antimicrobial activity of mucosal-associated invariant T cells. Nat Immunol. 11:701-708. PMID: 20581831
  • Pesnel S, Akkoul S, Ledée R, Leconge R, Pillon A, Kruczynski A, Harba R, Lerondel S, Le Pape A. 2011 Use of an image restoration process to improve spatial resolution in bioluminescence imaging. Mol Imaging. 10:446-452. PMID: 22201535
  • Vandamme, M., Robert, E., Lerondel, S., Sarron, V., Ries, D., Dozias, S., Sobilo, J., Gosset, D., Kieda, C., Legrain, B., Pouvesle, J.-M. & Pape, A.L. 2012. ROS implication in a new antitumor strategy based on non-thermal plasma. Int J Cancer. 130:2185-2194. PMID: 21702038
  • Guedj F, Lopes Pereira P, Najas S, Barallobre MJ, Chabert C, Souchet B, Sebrie C, Verney C, Herault Y, Arbones ML, Delabar JM. 2012. DYRK1A : a master regulatory protein controlling brain growth. Neurobiol Dis. 46: 190-203. PMID: 22293606
  • Allie N, Grivennikov SI, Keeton R, Hsu NJ, Bourigault ML, Court N, Fremond C, Yeremeev V, Shebzukhov Y, Ryffel B, Nedospasov SA, Quesniaux VF, Jacobs M. (2013)Prominent role for T cell-derived Tumour Necrosis Factor for sustained control of Mycobacterium tuberculosis infection. Sci Rep. May 9;3:1809. doi: 10.1038/srep01809.
National Centre for Biotechnology, CSIC, Madrid, Spain

CSIC logo

CNB-CSIC
Campus de Cantoblanco

C/ Darwin, 3
28049 Madrid
Spain

CNB Building

The National Centre for Biotechnology (CNB) belongs to the Spanish National Research Council (CSIC) and was inaugurated in 1992 with the aim of translating fundamental biological knowledge into tools and strategies applicable to human health, agriculture and environment, including the generation and analysis of genetically modified mice as experimental animal models of human diseases. The CNB annual budget is 42 million Euros with 172 projects executed in 2012, including 26 from the EC and 27 from biotech companies and private foundations, that resulted in 215 publications with a mean IF of 5,98. The CNB animal house (2000 m²/5000 cages/25000 mice) is divided into four areas (SPF, Conventional, Quaranteen and Inoculated/P3) and currently holds more than 350 different mouse strains. CNB scientific core facilities include a Transgenic Unit, shared with the CBMSO research centre, within Campus, led by Belén Pintado, and a Mouse Embryo and Sperm Cryopreservation and a Histology Unit, both directed by Lluis Montoliu. The Mouse Embryo Cryopreservation, Transgenic and Histology Facilities of CNB are integrated within the Scientific-Technological Platform INNOTEK, in support of research, belonging to the International Campus of Excellence of the Autonomous University of Madrid (UAM)+CSIC, coordinated from the CNB by Lluis Montoliu.

Key personnel

CNB-CSIC hosts the Spanish EMMA node since 2008, coordinated by Lluis Montoliu, Research Scientist CSIC and Honorary Professor of UAM, where he coordinates and teaches an annual Master Course on Genetic Modification of Mammals. Lluis Montoliu is the founder and President of the International Society for Transgenic Technologies (ISTT) and is also a member of the National CSIC Bioethics Committee. Lluis Montoliu has been involved in scientific projects involving animal transgenesis since 1991 and has been cryopreserving mouse embryos since 1993. The CNB Mouse Cryopreservation Unit has been in operation since 1999, with Julia Fernandez as the responsible senior technician. Julia Fernandez has been trained for mouse embryo and sperm cryopreservation techniques at the CNB, the Jackson Laboratory, the MRC-MGU/MLC Harwell (UK), the EMBL Transgenic Unit in Heidelberg and at the Italian EMMA node in Monterotondo, where Lluis Montoliu is also regularly appointed as instructor of the EMMA-JAX-CNR annual cryopreservation course. Julia Fernandez serves as instructor in the annual cryopreservation course organised by CIEMAT in Madrid since 2001 by Jorge Sztein (NIH) and Jesús Martínez-Palacio (CIEMAT). The cryopreservation facility at CNB has become a reference in the country and thus, frequently hosts technicians from other research centres, from Spain and elsewhere, willing to learn cryopreservation techniques to implement them in their institutes. Since 2013 the CNB-CBMSO Transgenic Facility is associated with the Spanish EMMA node and in charge of the mouse production tasks included within the Work-Package 8 of the new INFRAFRONTIER-I3 project, along with four additional EMMA nodes in Oulu, Vienna, Prague and Vari/Athens, under the coordination of Lluis Montoliu. In 2012, CNB-CSIC has signed a cooperation agreement with CNIO, the Spanish National Cancer Research Centre, in Madrid, to archive and distribute mutant mice of interest in biomedical research, generated by CNIO investigators, through the EMMA project and its Spanish node at the CNB-CSIC. Also during 2012, CSIC and the University of Kumamoto signed a cooperation agreement to promote the exchange of knowledge, personel and information on the mouse embryo and sperm cryopreservation and archiving activities undertaken by the Spanish EMMA node at CNB-CSIC and the CARD archive, coordinated in Japan by Prof. Naomi Nakagata.

Mouse resource enquiries: criocnb@cnb.csic.es

Selected references

  • Tiana M, Villar D, Pérez-Guijarro E, Gómez-Maldonado L, Moltó E, Fernández-Miñán A, Gómez-Skarmeta JL, Montoliu L, del Peso L. 2012. A role for insulator elements in the regulation of gene expression response to hypoxia. Nucleic Acids Res. 40:1916-27. PMID: 22067454
  • Martin D, Pantoja C, Fernández Miñán A, Valdes-Quezada C, Moltó E, Matesanz F, Bogdanović O, de la Calle-Mustienes E, Domínguez O, Taher L, Furlan-Magaril M, Alcina A, Cañón S, Fedetz M, Blasco MA, Pereira PS, Ovcharenko I, Recillas-Targa F, Montoliu L, Manzanares M, Guigó R, Serrano M, Casares F, Gómez-Skarmeta JL 2012. Genome-wide CTCF distribution in vertebrates defines equivalent sites that aid the identification of disease-associated genes. Nat Struct Mol Biol. 18:708-14. PMID: 21602820
  • Román AC, González-Rico FJ, Moltó E, Hernando H, Neto A, Vicente-Garcia C, Ballestar E, Gómez-Skarmeta JL, Vavrova-Anderson J, White RJ, Montoliu L, Fernández-Salguero PM. 2011. Dioxin receptor and SLUG transcription factors regulate the insulator activity of B1 SINE retrotransposons via an RNA polymerase switch. Genome Res. 21:422-32. PMID: 21324874
  • Zurita E, Chagoyen M, Cantero M, Alonso R, González-Neira A, López-Jiménez A, López-Moreno JA, Landel CP, Benítez J, Pazos F, Montoliu L. 2011. Genetic polymorphisms among C57BL/6 mouse inbred strains. Transgenic Res. 20:481-9. PMID: 20506040
  • Murillo-Cuesta S, Contreras J, Zurita E, Cediel R, Cantero M, Varela-Nieto I, Montoliu L. 2010. Melanin precursors prevent premature age-related and noise-induced hearing loss in albino mice. Pigment Cell Melanoma Res. 23:72-83. PMID:19843244
EMBL-EBI, Hinxton, United Kingdom

EBI Logo

EMBL-EBI
Wellcome Trust Genome Campus
Hinxton, Cambridgeshire, CB10 1SD
United Kingdom

EBI building

The European Molecular Biology Laboratory is a non-profit organisation and a basic research institute funded by 20 member states and one associate member (EMBL-Australia). Research at EMBL is conducted by approximately 85 independent groups covering the spectrum of molecular biology. The Laboratory has five units: the main Laboratory in Heidelberg, and Outstations in Hinxton (the European Bioinformatics Institute), Grenoble, Hamburg, and Monterotondo near Rome. The European Bioinformatics Institute (EBI) is located on the Wellcome Trust Genome Campus in Hinxton near Cambridge (UK). The EBI grew out of EMBL's pioneering work in providing public molecular databases and now maintains the world's most comprehensive range of freely available and up-to-date biological databases including DNA sequences (EMBL-Bank), protein sequences (UniProt), animal genomes (Ensembl), gene expression, (ArrayExpress) and mouse phenotype data from the International Mouse Phenotyping Consortium (IMPC). EBI is also a pivotal partner in ELIXIR, the European life sciences infrastructure for biological information, as part of the European Strategy on Research Infrastructures (ESFRI) process.

Key personnel

Helen Parkinson supervises the project at the EMBL-EBI. She leads the Samples, Phenotype and Ontologies team at the EMBL-EBI. Team activities focus on meta data integration, ontology development and supporting tooling, development and delivery of the EMBL-EBI's BioSample database and delivery of mouse data and supporting infrastructure for the biomedical research community. Project Leads Gautier Koscielny and Terry Meehan oversee the informatics for INFRAFRONTIER and ensure that developments are compatible with projects such as the International Knockout Mouse Consortium (IKMC) and the International Mouse Phenotyping Consortium (IMPC). Phil Wilkinson and Mike Relac are the primary EMBL-EBI developers of the INFRAFRONTIER website and the internal interfaces used by the INFRAFRONTIER partners for tracking of the archiving and supply of mice.

Selected references

  • Mallon AM, Iyer V, Melvin D, Morgan H, Parkinson H, Brown SD, Flicek P, Skarnes WC. Accessing data from the International Mouse Phenotyping Consortium: state of the art and future plans. Mamm Genome. 2012 Oct;23(9-10):641-52. doi: 10.1007/s00335-012-9428-9. Epub 2012 Sep 19. PMID: 22991088.
  • Murray SA, Eppig JT, Smedley D, Simpson EM, Rosenthal N. Beyond knockouts: cre resources for conditional mutagenesis. Mamm Genome. 2012 Oct;23(9-10):587-99. doi: 10.1007/s00335-012-9430-2. Epub 2012 Aug 29. 2012 Dec;23(11-12):791.  PMID: 22926223
  • Donahue LR, Hrabe de Angelis M, Hagn M, Franklin C, Lloyd KC, Magnuson T, McKerlie C, Nakagata N, Obata Y, Read S, Wurst W, Hörlein A, Davisson MT. Centralized mouse repositories. Mamm Genome. 2012 Oct;23(9-10):559-71. doi: 10.1007/s00335-012-9420-4. Epub 2012 Sep 4. PMID: 22945696
Helmholtz Center for Infection Research, Braunschweig, Germany

HZI logo

Dept. of Infection Genetics
Helmholtz Center for Infection Research
Inhoffenstr.7
38124 Braunschweig, Germany

HZI Building

The animal facilities at the Helmholtz Center for Infection Research (HZI) currently provide space for animal breeding with a total capacity of 19,000 cages, including a dedicated infection unit to perform experiments at the biosafety level BSL2. BSL3 animal laboratories will be in operation soon. Furthermore, all the necessary mouse technologies such as generation of Knock-Out strains, re-derivation of imported mouse strains by embryo transfer, freezing of mouse strains for storage, and in vitro fertilization are available.

Key personnel

Klaus Schughart is head of the Department of Infection Genetics at the HZI and holds a professorship at the University of Veterinary Medicine Hannover. He is an expert in the field of mouse genetics. He has established the ENU screen in mice at the Helmholtz Center in Munich and has performed phenotypic and genotypic characterization of several mouse mutants. In addition, he has experience in the genetic mapping and positional cloning of mouse mutations. His laboratory is analyzing traits that contribute to infection susceptibility to influenza A virus. His laboratory is participating in the German network "German Mouse Clinic" and the BMBF funded project „INFRAFRONTIER – Aufbau und Implementierung der deutschen Infrafrontier-Einrichtungen".Klaus Schughart is coordinator of the EU–funded COST network SYSGENET, a European network with 42 partners in 18 countries. This network is engaged in the field of complex genetics, systems biology and development of sophisticated experimental model systems for the better understanding and treatment of human diseases. He is a board member of the International Mammalian Genetics Society (IMGS) and a member of the Complex Trait Consortium (CTC).

Selected references

  • Schughart, K., Libert, C., and Kas, M.J. 2012. Human disease: Strength to strength for mouse models. Nature 492: 41. PMID: 23222600
  • T. Nedelko, H. Kollmus, F. Klawonn, S. Spijker, L. Lu, M. Heßman, R. Alberts, R. W. Williams, and K. Schughart. 2012. Distinct gene loci control the host response to influenza H1N1 virus infection in a time-dependent manner. BMC Genomics, 13: 411. PMID: 22905720
  • Pommerenke, C., Wilk E., Srivastava B., Schulze, A., Novoselova, N., Alberts, R., Geffers, R., and Schughart, K. 2012. Systematic analysis of the dynamics in gene expression changes describes the multi-level host responses during the course of an influenza A infection. PLoS ONE, 7(7):e41169.  PMID: 22815957
Helmholtz Zentrum München, Munich, Germany

Helmholtz Zentrum München
German Research Centre for Environmental Health GmbH
Ingolstädter Landstraße 1

85764 Neuherberg
Germany

 HMGU Building

The Helmholtz Zentrum München - German Research Centre for Environmental Health GmbH (HMGU) - located in Neuherberg / Munich - is a member of the Helmholtz Association, the largest research organisation in Germany. The Institute of Experimental Genetics (IEG) is interested in the functional analysis of mammalian genomes using the mouse as a model organism to carry out analyses of gene function and to elucidate the pathogenesis of diseases. Within a large scale ENU mutagenesis screen numerous new mutant lines were produced, leading to the identification of new genes and to valuable insight into gene function. In the German Mouse Clinic (GMC) standardized and comprehensive phenotypic analyses of mouse mutants from various sources are developed and offered to both partners from academia and industry. To manage the increasing number of mutants the expertise in cryopreservation and archiving has been built up since 1997, resulting in an archive of over 800 mutant mouse lines (sperm and embryos). Complemented with reproductive techniques, cryopreservation of germplasm provides a secure and economical management of storing the enormous numbers of new mutations generated enabling their use for future research.

Key personnel

Prof. Dr. Martin Hrabé de Angelis is head of the Institute of Experimental Genetics, Director of the German Mouse Clinic and since 2000 he is acting as the EMMA Director. He co-ordinated the INFRAFRONTIER preparatory phase project and InfraCoMP a project aiming to coordinate the activities of IMPC and INFRAFRONTIER. Furthermore, he is a member of the IMPC Steering Committee and participates in the IMPC Industry Work Group. Dr. Michael Hagn, MBA and Dr. Sabine Fessele, PMP are the EMMA project managers running the EMMA Project Office since 2005. Dr. Susan Marschall, responsible for the Cryo-Unit of the IEG, has worked in the field of cyropreservation for many years. Dr. Michael Räß, MSc. and Dr. Ana de Castro are project managers of the INFRAFRONTIER Research Infrastructure and of the InfraCoMP project. Dr. Valerie Gailus Durner and Dr. Helmut Fuchs are the scientific-administrative and scientific-technical heads of the German Mouse Clinic since 2001.

Mouse resource enquiries: emma-munich@infrafrontier.eu

Selected publications

  • Beckers, J., W. Wurst, and M.H. de Angelis. 2009. Towards better mouse models: enhanced genotypes, systemic phenotyping and envirotype modelling. Nat Rev Genet 10:371-380. PMID: 19434078
  • Enard, W., S. Gehre, K. Hammerschmidt, S.M. Holter, T. Blass, M. Somel, M.K. Bruckner, C. Schreiweis, C. Winter, R. Sohr, L. Becker, V. Wiebe, B. Nickel, T. Giger, U. Muller, M. Groszer, T. Adler, A. Aguilar, I. Bolle, J. Calzada-Wack, C. Dalke, N. Ehrhardt, J. Favor, H. Fuchs, V. Gailus-Durner, W. Hans, G. Holzlwimmer, A. Javaheri, S. Kalaydjiev, M. Kallnik, E. Kling, S. Kunder, I. Mossbrugger, B. Naton, I. Racz, B. Rathkolb, J. Rozman, A. Schrewe, D.H. Busch, J. Graw, B. Ivandic, M. Klingenspor, T. Klopstock, M. Ollert, L. Quintanilla-Martinez, H. Schulz, E. Wolf, W. Wurst, A. Zimmer, S.E. Fisher, R. Morgenstern, T. Arendt, M.H. de Angelis, J. Fischer, J. Schwarz, and S. Paabo. 2009. A humanized version of Foxp2 affects cortico-basal ganglia circuits in mice. Cell 137:961-971. PMID: 19490899
  • Fuchs, H., V. Gailus-Durner, T. Adler, J.A. Pimentel, L. Becker, I. Bolle, M. Brielmeier, J. Calzada-Wack, C. Dalke, N. Ehrhardt, N. Fasnacht, B. Ferwagner, U. Frischmann, W. Hans, S.M. Holter, G. Holzlwimmer, M. Horsch, A. Javaheri, M. Kallnik, E. Kling, C. Lengger, H. Maier, I. Mossbrugger, C. Morth, B. Naton, U. Noth, B. Pasche, C. Prehn, G. Przemeck, O. Puk, I. Racz, B. Rathkolb, J. Rozman, K. Schable, R. Schreiner, A. Schrewe, C. Sina, R. Steinkamp, F. Thiele, M. Willershauser, R. Zeh, J. Adamski, D.H. Busch, J. Beckers, H. Behrendt, H. Daniel, I. Esposito, J. Favor, J. Graw, G. Heldmaier, H. Hofler, B. Ivandic, H. Katus, M. Klingenspor, T. Klopstock, A. Lengeling, M. Mempel, W. Muller, S. Neschen, M. Ollert, L. Quintanilla-Martinez, P. Rosenstiel, J. Schmidt, S. Schreiber, K. Schughart, H. Schulz, E. Wolf, W. Wurst, A. Zimmer, and M. Hrabe de Angelis. 2009. The German Mouse Clinic: a platform for systemic phenotype analysis of mouse models. Curr Pharm Biotechnol 10:236-243. PMID: 19199957
Instituto Gulbenkian de Ciência, Oeiras, Portugal

FCG logo

Instituto Gulbenkian de Ciência
Rua da Quinta Grande, 4
2780-901 Oeiras
Portugal

FCG building

The Instituto Gulbenkian de Ciência (IGC) was founded and is supported by the Fundação Calouste Gulbenkian to carry out biomedical research and education. The institute's scientific interests are focused on the genetic basis of development and the evolution of complex systems and concentrates on organism-centred approaches in experimental models that include plants, yeast, flies and mice, and on the genetics of complex human diseases. The IGC mouse facility is partitioned into 5 independent areas, each with a specific bio-containment level, including germ-free and strict SPF. The facility hosts about 30,000 mice and maintains up to 100 different inbred strains, which are available to in-house and associated research groups, and to visiting scientists. Production and experimental areas are set to serve active research in various fields of biology (development, neurobiology, infectious diseases, immunology, genetics). The facility provides services for the generation of transgenic and knock-out mice, embryo re-implantation, embryo and sperm freezing. In the frame of INFRAFRONTIER and EMMA. The IGC offers the Germ-Free Service that generates, breeds and houses mice that are free of all microorganisms. These germ-free animals are crucial in studies aimed at understanding the effects of microorganisms on a host, or dissecting the molecular mechanisms underlying the function of the immune system.

Key personnel

Jocelyne Demengeot, Principal Investigator, head of the Animal Facility and Deputy Director for Science is representing Portugal in the EMMA Board of Participating Directors.

Mouse resource enquiries: emmaportugal@igc.gulbenkian.pt

Selected references

  • Hirota K, Turner JE, Villa M, Duarte JH, Demengeot J, Steinmetz OM, Stockinger B. 2013 Plasticity of TH17 cells in Peyer's patches is responsible for the induction of T cell-dependent IgA responses. Nat Immunol.  14:372-9.
    PMID: 23475182
  • Zelenay S, Bergman ML, Paiva RS, Lino AC, Martins AC, Duarte JH, Moraes-Fontes MF, Bilate AM, Lafaille JJ, Demengeot J.2010 Cutting edge: Intrathymic differentiation of adaptive Foxp3+ regulatory T cells upon peripheral proinflammatory immunization.J Immunol. 185:3829-33.
    PMID: 20817879
  • Demengeot J, Zelenay S, Moraes-Fontes MF, Caramalho I, Coutinho A. 2006. Regulatory T cells in microbial infection.Springer Semin Immunopathol.  Aug;28(1):41-50
Institute of Molecular Genetics ASCR, Prague, Czech Republic

 

Institute of Molecular Genetics ASCR
Videnska 1
083142 20 Prague
Czech Repulic

IMG building

The Institute for Molecular Genetics (IMG) currently hosts 24 research groups with over 200 scientists dealing with topics covering molecular and cellular immunology, molecular and cellular oncology, cell biology of the nucleus and cytoskeleton, functional genomics and bioinformatics, study of oncogenes, molecular biology of development, structural biology and mechanisms of receptor signaling. IMG hosts the largest collection of unique genetically defined mouse strains in the Czech Republic comprising more than 280 strains today. To support mouse genetic research and to manage generation, archiving, and distribution of the mouse models IMG established a new transgenic core facility (TgU) in 2008. TgU offers all up-to-date techniques for embryo-manipulation, including generation of genetically altered mice, rederivation, archiving, distribution, and consulting. TgU was established by R.Sedlacek who heads the core facility and manages the Czech nodes of EMMA and INFRAFRONTIER. IMG is partner of the project BIOCEV. This is a joint project of six institutes of the Academy of Sciences of the Czech Republic and two faculties of Charles University in Prague. The project's goal is to establish a European Centre of Excellence in biomedicine and biotechnology.

Key personnel

Prof. Radislav Sedlacek, is currently head of the Dept. of Transgenic Models of Diseases and the Transgenic Unit, project director of the Czech Centre for Phenogenomics (BIOCEV), and a coordinator of BMS infrastructures of the National Roadmap. His professional background: originally immunologist/biochemist, later transgenic specialist. Prof. Radislav Sedlacek generated and analyzed a number of mouse models to reveal functions of proteolytic enzymes. Prof. Radislav Sedlacek represents IMG in EMMA and INFRAFRONTIER. Prof. Jiri Forejt, is currently head of the Dept. of Mouse Molecular Genetics with a professional background in Genetics and genomics of the laboratory mouse. His reseach covers meiotic sex chromosome inactivation as well as genetic architecture of hybrid sterility in mouse (Mus m. musculus x Mus m. domesticus) and experimental introgression of musculus chromosomes into domesticus genetic background.

Mouse resource enquiries: emma-ccp-prague@img.cas.cz

Selected references

  • Bhattacharyya T, Gregorova S, Mihola O, Anger M, Sebestova J, Denny P, Simecek P, Forejt J. 2013.Mechanistic basis of infertility of mouse intersubspecific hybrids. Proc Natl Acad Sci USA. PMID: 23329330
  • Fafilek B., Krausova M, Vojtechova M, Pospichalova V, Tumova L, Sloncova E., Huranova M., Chmelikova  J., Hlavata A., Svec J., Sedlacek R., Luksan O., Oliverius M, Voska L., Jirsa M., Paces J., Kolar M., Krivjanska M., Klimesova K., Tlaskalova-Hogenova H. and Korinek V. 2012. Troy, a Tumor Necrosis Receptor Family Member 19, Interacts with Lgr5 to Inhibit Wnt Signaling in Intestinal Stem Cells. Gastroenterology. 7:01607-1 PMID: 23142137
  • Jirouskova M., Zbodakova O., Gregor M., Chalupsky K, Sarnova L., Hajduch M, Ehrmann J., Jirkovska M., and Sedlacek R. 2012. Hepatoprotective effect of MMP-19 deficiency in a mouse model of chronic liver fibrosis. PLoS One. 7. doi: 10.1371/journal.pone.0046271.
    PMID: 23056273
Karolinska Institute, Stockholm, Sweden

 

KI logo

Karolinska Institutet
Dept. Comparative Medicine, KCTT
von Eulers väg 4a, 17177 Stockholm
Sweden

The Karolinska Institute (KI) is a leading medical university in Europe, with approximately 3000 employees, and carries out front-line research in many areas of medicine and biology. KI has a large core facility for production, cleansing and archiving of genetically modified mice. This unit, the Karolinska Centre for Transgenic Technology (KCTT), belongs to the Department of Comparative Medicine, headed by Prof. Brun Ulfhake. KCTT also organizes international conferences, most importantly the International Series of Transgenic Technology Meetings.

Key personnel

Prof. Urban Lendahl is director of two research centres (DBRM and WIRM) and did originally start the transgenic core facility at KI. He is Professor of Genetics at the Department for Cell and Molecular Biology at the KI. Dr. Johannes Wilbertz was recruited to KI from a position as head of an MPI transgenic unit in Heidelberg and was the former head of KCTT. He is head of the new Wallenberg Research Facility, coordinator of the different core facilities within the new facility and member of the steering committee of the Comparative Medicine at KI. Dr. Wilbertz has a strong background in transgenic technology, mouse husbandry, facility planning, archiving and animal ethics/laws. Zuzana Khoshidi is the local coordinator for EMMA at the KCTT and has been involved in EMMA since the establishment of the EMMA node at the KI. Dr. Stephan Teglund is the present head of the KCTT and responsible for supervising all activities. Dr. Olga Lund is the head of the cryobank.

Mouse resource enquiries: kctt-cryo@km.ki.se

Selected references

  • Hansson, E.M., Lanner, F., Das, D., Mutvei, A., Marklund, U., Ericson, J., Farnebo, F., Stumm, G., Stenmark, H. Andersson, E.R. and Lendahl, U. 2010. Control of Notch ligand endocytosis by Mind bomb and ligand-receptor interaction. Journal of Cell Science 123: 2931-2942. PMID: 20720151
  • Lanner F, Lee KL, Sohl M, Holmborn K, Yang H, Wilbertz J, Poellinger L, Rossant J, Farnebo F. 2010. Heparan Sulfation Dependent FGF Signalling Maintains Embryonic Stem Cells Primed for Differentiation in a Heterogeneous State. Stem Cells 28:191-200. PMID: 19937756
  • Landor, S., Mutvei, A., Mamaeva, V., Jin, S., Busk, M., Borra, R., Grönroos, T.J., Kronqvist, P., Lendahl, U. and Sahlgren, C. 2011. Hypo- and hyperactivated Notch signaling induce a glycolytic switch through distinct mechanisms. Proc. Natl. Acad. Sci. USA 108, 18814-18819 PMID: 22065781
KU Leuven, Leuven, Belgium

KULeuven logo

Laboratory of Molecular Biology (Celgen)
Department Development and Regeneration
KU Leuven Campus Gasthuisberg

Building Ond&Nav4 - p.o.box 812
Stem Cell Institute
Herestraat 49
3000 Leuven
Belgium

KULeuven Building

Our team studies in collaboration with international leaders in their field a number of in-house identified components of TGFβ family signaling. The main strength of our work is the successful combination of (i) functional analysis in the vertebrate embryo (mainly using conditional knockout (cKO) mice) and cell cultures (including stem/progenitor cells in the mouse embryo) with (ii) biochemical studies (protein-protein and protein-DNA interaction, transcriptomics and proteomics) addressing the action mechanism(s) of the studied proteins. Using a follow-the-phenotype approach the in vivo work has taken us into Nodal/Activin and BMP signaling, ranging from early post-gastrulation embryogenesis to somitogenesis, embryonic hematopoiesis, angiogenesis, cardiac morphogenesis, neural crest biology and its relation to craniofacial development, various aspects of peripheral and central nervous system (CNS) development including studies on neurogenesis and gliogenesis in the cortex, GABAergic interneuron migration, and myelination from oligodendrocyte precursor cells, and to adult neurogenesis, and neurodegeneration and regeneration. The animal model used was chiefly mouse. Doing so, it has become clear that the components we study, including a number of Smad-interacting proteins, regulate diverse molecular and cellular processes and are also directly relevant to development and disease. While this type of work on individual genes will continue, we want to shift it more towards work addressing gene function post-natally, including in adult somatic stem cells and cells of their niche, with emphasis on developmental signaling and its nuclear interpretation, in neurodevelopment and neuropathology. This follows the internationally recognizable trend that embryologists, seen the medical relevance of their findings, move into human diseases and as part of their studies decide to model aspects of these pathologies in animals. In addition, because of the rapid developments in the field it is essential also for us to take such studies in the mouse increasingly to stem/progenitor cells studies ex vivo, with emphasis – ideally again guided by the phenotypes seen in vivo – on cell fate commitment and differentiation, including in human ESCs and iPSCs.  Currently we coordinate in Flanders the large infrastructure Hercules Foundation project InfraMouse, with focus on mouse production, cryopreservation and re-derivation, and developmental and adult phenotyping in the nervous systems and in the blood vessel wall. An extension and expansion into InfraMouse2.0 has now been proposed to the funding bodies and the government such that Belgium can also join the ESFRI roadmap of INFRAFRONTIER in the future.

Key personnel

Danny Huylebroeck is member of the Project Management Committee of INFRAFRONTIER and has over many years - on request by the European Commission or on invitation by the project coordinators - attended multiple progress and review meetings of the projects like EUCOMM, EUMODIC, SyBoSS, EUCOMMTOOLS, InfraCOMP, and hence also IKMC and IMPC.

Selected references

  • van den Berghe V, Stappers E, Vandesande B, Dimidschstein J, Kroes R, Francis A, Conidi A, Lesage F, Dries R, Cazzola S, Berx G, Kessaris N, Vanderhaeghen P, van Ijcken W, Grosveld FG, Goossens S, Haigh JJ, Fishell G, Goffinet A, Aerts S, Huylebroeck D*, Seuntjens E*. Directed migration of cortical interneurons depends on the cell-autonomous action of Sip1. Neuron. 77: 70-82. PMID: 23312517
  • Weng Q, Chen Y, Wang H, Xu X, Yang B, He Q, Shou W, Chen Y, Higashi Y, van den Berghe V, Seuntjens E, Kernie SG, Bukshpun P, Sherr EH, Huylebroeck D, Lu QR*. Dual-mode modulation of Smad signaling by Smad-interacting protein Sip1 is required for myelination in the central nervous system. Neuron. 2012. 73:713-28. PMID: 22365546
  • Moya IM, Umans L, Maas E, Pereira PN, Beets K, Francis A, Sents W, Robertson EJ, Mummery CL, Huylebroeck D, Zwijsen A*. Stalk cell phenotype depends on integration of Notch and Smad1/5 signaling cascades. Dev Cell. 13: 501-14. PMID: 22364862
MRC Harwell, Oxfordshire, United Kingdom

MRC Logo

MRC Harwell
Harwell Science and Innovation Campus
Oxfordshire
OX11 0RD
UK

MRC building

 

The MRC Harwell consists of the Mammalian Genetics Unit (MGU) and the Mary Lyon Centre (MLC). Both units provide an integrated campus of mouse genetics and genomics, which partner extensively with academia and industry. As a major international research centre, the MGU is at the forefront of mouse genetics and functional genomic research. With the support of large scale gene targeting and ENU mutagenesis functional genomics platforms, the MGU undertakes a range of research programmes focused on enhancing our understanding of the molecular and genetic bases of human disease in lifetime studies from development to ageing. The MLC is an international facility for mouse functional genomics providing services ranging from clinical chemistry, imaging, histology, through to gene targeting and archiving mouse lines. The MLC has also developed a wide range of sophisticated phenotyping platforms and hosts the MRC Frozen Embryo and Sperm Archive (FESA) which offers >1200 novel mouse strains to the scientific community. MRC Harwell also has wide-ranging expertise in developing innovative on-line mouse datasets. These include the phenotyping portals supporting the IMPC Data Coordination Centre, as well as developing analysis and reporting tools to support data curation and integration. The MouseBook databases and web portal provide access to information about mutant mouse lines held as live or cryopreserved stocks at MRC Harwell.

MRC Harwell is a founding member of the European Mouse Mutant Archive (EMMA) and of the International Mouse Phenotyping Consortium (IMPC), which is currently funded by the NIH and MRC to produce and phenotype 600 knock-out mouse lines. MRC Harwell was the coordinator of the EC-funded EUMORPHIA consortium (European Union Mouse Research for Public Health and Industrial Applications). EUMORPHIA led to the foundation of EUMODIC, which undertook the primary phenotype assessment of 500 mouse lines in a pilot to the IMPC. Furthermore, MRC Harwell was a member of the European Conditional Mouse Mutagenesis Project (EUCOMM).

Key personnel

Prof. Steve D.M. Brown is the director of the Mammalian Genetics Unit, Dr. Sara Wells is the director of the Mary Lyon Centre and Dr Martin Fray is the head of biological resources at MLC.

Mouse resource enquiries: FESA@har.mrc.ac.uk
Phenotype enquiries: IMPC@har.mrc.ac.uk

Selected references

  • Nolan PM, Peters J, Strivens M, Rogers D, Hagan J, Spurr N, Gray IC, VIZOR L, Brooker D, Whitehill E, Washbourne R, Hough T, Greenaway S, Hewitt M, Liu X, McCormack S, Pickford K, Selley R, Wells C, Tymowska-Lalanne Z, Arkell R, Mburu P, Hardisty R, Kiernan A, Ervem A, Steel KP, Vogeling S, Guenet JL, Nickols C, Sadri R, Naase M, Isaacs A, Davies K, Browne M, Fisher EMC, Martin J, Rastan S, Brown SDM and Hunter J. (2000) A systematic genome-wide phenotype-driven mutagenesis programme for gene function studies in the mouse. Nature Genetics 25: 440-443 PMID: 10932191
  • Brown SDM, Hrabe de Angelis M, Chambon P and the EUMORPHIA Consortium. (2005) EMPRESS: standardised phenotype screens for functional annotation of the mouse genome. Nature Genetics 37: 1155 PMID: 16254554
  • Guan M, Marschall S, Raspa M, Pickard AR, Fray MD. (2012) Overview of new developments in and the future of cryopreservation in the laboratory mouse. Mammalian Genome 23: 572-9. PMID: 22936001
Netherlands Cancer Institute, Amsterdam, The Netherlands

 MCCA Logo

The Netherlands Cancer Institute (NKI)
Plesmanlaan 121
1066 CX Amsterdam
The Netherlands

NKI building

The Netherlands Cancer Institute (NKI) is an integrated cancer institute, combining hospital and research laboratory under one roof. The research laboratory covers all major areas of oncology research, with special emphasis on mouse tumor models, cell based screens, and translational research. The institute has excellent facilities, including a radioactivity lab, flow cytometry, confocal-, FRET-, and electron- microscopy, a microarray and deep-sequencing facility, a robotics facility for high-throughput screening and a large bioinformatics group. The NKI has extensive expertise in generating genetically modified mouse strains and production of genetically engineered mouse models (GEMMs) that closely reproduce tumors as found in man. The institute has a large mouse facility (capacity 20,000 cages) and a core facility for the generation of genetically engineered mice. The institute has ample expertise in mouse pathology and an established record in studying drug resistance mechanisms in mouse models of human cancer. The NKI has been at the forefront of several key innovations in cancer genetics such as large-scale gene inactivation through RNA interference to identify the function of novel cancer-related genes and the conditional knockout mouse technology, allowing rapid in vivo validation of candidate cancer genes and drug targets. To accelerate the development of novel anti-cancer therapeutics, the NKI has established a Mouse Clinic for Cancer and Aging research (MCCA), which is supported by a 18.6M Euro grant from the National Roadmap Committee for Large-scale Research Facilities.The MCCA houses three key expertises: 1. A pipeline for accelerated production of novel GEMMs based on rapid and reproducible introduction of gain-of-function or loss-of-function alleles into embryonic stem cells (ESCs) derived from existing GEMMs. The modified GEMM-ESCs can be used for direct production of experimental cohorts of chimeric mice or for production of F1 animals by breeding the chimeras with the original GEMM;  2. A repository for distribution of established and validated GEMM-ESC lines as part of the European Mutant Mouse Archive (EMMA) project and INFRAFRONTIER-I3. a fully equipped Mouse Cancer Clinic for preclinical intervention studies and imaging of cancer growth and therapy response in GEMMs of human cancer.The NKI participates in the FP7 EurocanPlatform project.

Key personnel

Jos Jonkers, Ivo Huijbers

Selected references

  • Huijbers IJ, Bin Ali R, Pritchard C, Cozijnsen M, Proost N, Song, JY, de Vries H, Badhai J, Sutherland K, Krimpenfort P, Michalak E, Jonkers J, Berns A. 2013. Speedy generation of cancer mouse models with re-derived embryonic stem cells. Submitted.
  • Huijbers IJ, Krimpenfort P, Berns A, Jonkers J. 2011. Rapid validation of cancer genes in chimeras derived from established genetically engineered mouse models. Bioessays. 33: 701-10. PMID: 21735458
  • van Miltenburg MH, Jonkers J. 2012. Using genetically engineered mouse models to validate candidate cancer genes and test new therapeutic approaches. Curr Opin Genet Dev. 22: 21-7. PMID: 22321988
Sanger Institute, Hinxton, United Kingdom

Sanger logo

Wellcome Trust Sanger Institute,
Wellcome Trust Genome Campus,
Hinxton, Cambridge CB10 1SA
UK

WTSI Building

The Sanger Institute is a non-profit research institute dedicated to the structural and functional characterization of the genome of several species of biomedical importance, including the human and the mouse. The Mouse Genetics Project is a flagship research project of the Institute dedicated to generate, characterize, archive, and distribute mutant mouse strains developed from the EUCOMM / KOMP resources. Mutant mice are generated in a large scale, high-throughput manner (>200/yr). At least 160 strains are subject to a standard phenotypic analysis per year. All the data and mouse resources are freely available to the community. Embryos and / or sperm for each of the strains are frozen and deposited with other partners of the EMMA repository or with the KOMP repository at UC Davis. Distribution of live mice directly to the end-user also occurs from the Sanger Institute if the germplasm has not yet been archived.

Key personnel

The Head of Mouse Pipelines for the Sanger Institute Mouse Genetics Project (Sanger MGP) leads the operational aspects of multiple mouse production and phenotyping  pipelines and represents the mouse interests of the Sanger Institute in several international consortia and projects: EUMODIC, EUCOMM, EUCOMMTOOLS, CREATE, INFRAFRONTIER-I3, EMMAservice, KOMP2, InfraCoMP and IMPC. The Mouse Production Manager has responsibility for the Transgenic Technologies team which includes the activities of blastocyst and pronuclear microinjection, embryo and sperm cryopreservation, and in vitro fertilization, as well as responsibility for research, development and implementation of innovative techniques and technologies. The Project and Distribution Manager has responsibility for overall project management as well as being principle coordinator of mouse strain distribution to the scientific community and to the repositories. The Mouse Phenotyping Manager has responsibility for the Sanger MGP phenotyping team which includes In-life, Developmental Biology, and Infection and Immunology.

Mouse resource enquiries: mouseinterest@sanger.ac.uk
Phenotype enquiries: MGPEnquiries@sanger.ac.uk
MTA enquiries: mousemta@sanger.ac.uk

Selected references

  • Ryder E et al. (2014). Rapic conversion of EUCOMM/KOMP-CSD alleles in mouse embryos using a cell-permeable Cre recombinase. Transgenic research, 23(1), 177-85. PMID:24197666
  • JK White et al. (2013) Genome-Wide Generation and Systematic Phenotyping of Knockout Mice Reveals New Roles for Many Genes. Cell. 154(2):452-64. PMID:23870131
  • Ryder E, et al. (2013) Genomic analysis of a novel spontaneous albino C57BL-6N mouse strain. Genesis, 51(7), 523-528. PMID:23620107
  • Ryder E, et al. (2013) Quality control assessment of 731 mouse lines produced using the EUCOMM/KOMP-CSD embryonic stem cell resource. Mammalian Genome, 24(7-8), 286-294. PMID:23912999
Tel Aviv University, Tel Aviv, Israel

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Tel Aviv University
P.O. Box 39040
Tel Aviv 6997801
Israel

Tel Aviv University is the largest comprehensive research university in Israel. Comprising 9 faculties, 24 Schools and 105 departments, the university has over one hundred research centers and institutes. On top of the extensive range of study programs that TAU offers its 29,000 students, over the years Tel Aviv University has introduced an ever-increasing number of interdisciplinary programs in vital new fields such as nanoscience and nanotechnology, bioinformatics and biotechnology. Among the university's 1,000 faculty members are internationally renowned scientists who have made a major contribution to their respective fields. In 2012 Tel Aviv University was ranked 16th in the world in the criteria citations per faculty member. Tel Aviv University participates in the European Framework Programmes since the 5th Framework Programme. With participation in 50 projects in FP5 and 75 projects in FP6. Since the beginning of FP7, Tel Aviv University is part of over 160 EC funded projects, several of them as the coordinator. The Faculty of Medicine at TAU conducts an outstanding research on the preclinical and clinical areas. The INFRAFRONTIER project is under the mandate of the faculty of Medicine at TAU. Two mouse facilities (conventional and SPF) are at the faculty of Medicine with a capacity of more 6000 and 4000 cages at the conventional and SPF facilities, respectively.

Key personnel

Prof. Fuad A. Iraqi is the Head of the INFRAFRONTIER project in Israel. He is also a member of the management committee of two cooperation of science and technology consortiums (COST) in Europe, including SYSGENET, for studying system genetics in the mouse model and GENIEUR, for studying irritable bowel syndrome (IBS). Prof. Iraqi is leading the development of the collaborative cross (CC) mouse genetic reference (GRP) population in Israel.

Selected references

  • Churchill G, Airey D, Allayee H, Angel J, Attie A, Beatty J, Beavis W, Belknap J, Bennett B, Berrettini W, Bleich A, Bogue M, Broman K, Buck K, Buckler E, Burmeister M, Chesler E, Cheverud J, Clapcot, S, Cook ., Cox R, Crabbe J, Crusio W, Darvasi A, Deschepper C, Doerge R, Farber C, Forejt J, Gaile D, Garlow S, Geiger H, Gershenfeld H, Gordon T, Gu J, Gu W., de Haan G, Hayes N, Heller C, Himmelbauer H, Hitzemann R, Hunter K, Hsu H, Iraqi FA, Ivandic B, Jacob H, Jansen R, Jepsen K, Johnson D, Johnson T, Kempermann G, Kendziorski C, Kotb M, Kooy R, Llamas B, Lammert F, Lassalle J, Lowenstein P, Lu L, Lusis A, Manly K, Marcucio R, Matthews D, Medrano J, Miller D, Mittleman G, Mock B, Mogil J, Montagutelli X, Morahan G, Morris D, Mott R, Nadeau J, Nagase H, Nowakowski R, O'Hara B, Osadchuk A, Page G, Paigen B, Paigen K, Palmer A, Pan H, Peltonen-Palotie L, Peirce J, Pomp D, Pravenec M, Prows D, Qi Z, Reeves R, Roder J, Rosen G, Schadt E, Schalkwyk L, Seltzer Z, Shimomura K, Shou S, Sillanpää M, Siracusa L, Snoeck H, Spearow J, Svenson K, Tarantino L, Threadgill D, Toth L, Valdar W, de Villena F, Warden C, Whatley S, Williams R, Wiltshire T, Yi N, Zhang D, Zhang M, Zou F. 2004. Complex Trait Consortium. The Collaborative Cross, a community resource for the genetic analysis of complex traits. Nat Genet 36: 1133-1137. PMID: 15514660.
  • Iraqi FA, Gary Churchill and Mott R. 2008. The Collaborative Cross, developing a resource for mammalian systems genetics: A status report of the Wellcome Trust cohort. Mamm. Genome 19: 379-381. PMID: 18521666 
  • Aylor DL, William V, Wendy FMW, Buus RJ, Ricardo A. Verdugo RA, Ralph S. Baric RS, Ferris MT, Frelinger FA, Heise M, Frieman MB, Gralinski LE, Bell TA, Didion JP, Hua K, Nehrenberg DL, Powell CL, Steigerwalt J, Xie Y, Kelada SNP, Collins FS, Yang IV, Schwartz DA, Branstetter LA, Chesler EJ, Miller DR, Spence J, Liu EY, McMillan L, Sarkar A, Wang J, Wang W, Zhang Q, Broman KW, Korstanje R, Durrant C, Mott R, Iraqi FA, l Pomp D, Threadgill D, de Villena FPM, Churchill GA. 2011. Genetic Analysis of Complex Traits in the Emerging Collaborative Cross. Genome Research 21(8): 1213-1222. PMID: 21406540
Toronto Centre for Phenogenomics, Toronto, Canada

TCP logo

Toronto Centre for Phenogenomics
25 Orde Street
Toronto ON Canada M5T 3H7

TCP building

The Toronto Centre for Phenogenomics opened in October 2007. It is a shared infrastructure between two major research hospitals ('Member Hospitals') and external partners that operates as a centralized, state-of-the-art mouse facility, with the goal of improving human health. The TCP is the largest centre of its kind in Canada, and achieves excellence with economies of scale. The building is approximately 11,000 gross m2 of space dedicated to specialized laboratories for mouse generation, holding and procedure space to support individual investigator-driven research projects, and large-scale programs. The TCP is functionally programmed to pool expertise and facilities under one roof to conduct and support research involving generation of mutant mice, multi-organ and disease system baseline and in-depth phenotyping, pathology, multimodality imaging, and cryopreservation for storage and distribution. Three research programs located at the TCP are dedicated to mouse production, phenotyping, archiving, and distribution. The high-throughput production and phenotyping program provides ES cell-to-mouse production and a comprehensive pipeline that supports the research requirements of academic users and industry partners to screen mutants for phenotypes and more fully characterize mutants under study. This program conducts the NorCOMM2 project to produce and phenotype knockout mouse lines from IKMC ES cells as a member of the International Mouse Phenotyping Consortium (IMPC) and is one of three sites of the NIH KOMP2 DTCC consortium, also a part of the IMPC.The Mouse Imaging Centre (MICe) provides collaborative access to state-of-the-art digital imaging technologies, including magnetic resonance microscopy (MRM), micro-computed tomography, and optical projection tomography for the morphological analysis of mutant mice.The Canadian Mouse Mutant Repository (CMMR) provides cryopreservation, in vitro fertilization, and multiple format tissue archiving and distribution. It is the repository for the NorCOMM, NorCOMM2, and NorCOMM2LS Projects. NorCOMM is Canada's contributions to the International Knockout Mouse Consortium (IKMC) that is developing and distributing a library of ES cell lines carrying single gene mutations across the mouse genome. The NorCOMM2LS Project is led at the CMMR and is partnered with EUCOMMTOOLS to produce a comprehensive Cre-driver resource for use by the community and with collaborators at the University of Manitoba for production of de novo targeted ES cell lines to the IKMC.

Key personnel

Colin McKerlie

Selected references

  • Adams D, Baldock R, Bhattacharya S, Copp AJ, Dickinson M, Greene NDE, Henkelman M, Justice M, Mohun T, Murray SA, Pauws E, Raess M, Rossant J, Weaver T, West D. Bloomsbury report on mouse embryo phenotyping: Recommendations from the IMPC Workshop on Embryonic Lethal Screening. (in press) Disease Models Mech.
  • Donahue LR, Hrabé de Angelis M, Hagn M, Franklin C, Lloyd KC, Magnuson T, McKerlie C, Nakagata N, Obata Y, Read S, Wurst W, Hörlein A, Davisson MT. 2012. Centralized mouse repositories. Mamm Genome 23: 559-571. PMID: 22945696
  • Bradley A, Anastassiadis K, Ayadi A, Battey JF, Bell C, Birling M-C, Bottomley J, Brown SDM, Bürger A, Bult CJ, Bushell W, Collins FS, Desaintes C, Doe B, Economides A, Eppig JT, Finnell RH, Fletcher C, Fray M, Frendewey D, Friedel RH, Grosveld FG, Hansen J, Hérault Y, Hicks G, Hörlein A, Houghton R, Hrabé de Angelis M, Huylebroeck D, Iyer V, de Jong PJ, Kadin JA, Kaloff C, Kennedy K, Koutsourakis M, Lloyd KCK, Marshall S, Mason J, McKerlie C, McLeod MP, von Melchner H, Moore M, Mujica AO, Nagy A, Nefedov M, Nutter LM, Pavlovic G, Peterson JL, Pollock J, Ramirez-Solis R, Rancourt DE, Raspa M, Remacle JE, Ringwald M, Rosen B, Rosenthal N, Rossant R, Ruiz Noppinger P, Ryder E, Schick JZ, Schnütgen F, Schofield P, Seisenberger C, Selloum M, Simpson EM, Skarnes WC, Smedley D, Stanford WL, Stewart AF, Stone K, Swan K, Tadepally H, Teboul L, Tocchini-Valentini GP, Valenzuela D, West AP, Yamamura K, Yoshinaga Y, Wurst W. (2012) The mammalian gene function resource: the international knockout mouse consortium. Mamm Genome 23 (9-10): 580-586. PMID: 22968824
TSE Systems GmbH, Bad Homburg, Germany

TSE logo

TSE Systems GmbH
Siemensstr. 21
61352 Bad Homburg
Germany

The company's roots can be traced back to 1886, when it was established in Thuringia, Germany. From the early beginnings TSE Systems has developed into a leading supplier of sophisticated research instrumentation in the global life science market. The company's focus is on providing the total customer solution, with modular designs of integrated hardware and software platforms for laboratory animal research such as neuroscience, phenotyping, drug screening and toxicology. In more than 125 years of tradition and experience, TSE Systems has acquired a vast portfolio of technologies and core competencies. Moreover, TSE Systems has highly qualified specialists together with first-class facilities for mechanical and electronic design as well as for production and software development in accordance with quality assurance systems such as ISO 9000 and customer-adapted GLP requirements. This is one of the main reasons why TSE Systems has developed to become the leading supplier of high-quality phenotyping systems – both classical and automated high-throughput systems for small laboratory animals. The objective of the TSE project work is the Metabolic PhenoCage (MPC) which, in addition to recording "classical" metabolic parameters, also includes the automatic separation and quantification of urine and feces. In the context of the INFRAFRONTIER-I3 project the MPC is being optimized to match the ever-increasing research requirements. Recording the metabolic parameters as well as the activity parameters is being refined with respect to the special MPC design. The measuring tools are being checked for their usability and, if necessary, redesigned to increase their high-throughput capabilities and user friendliness. In addition to the scientific / technical aspects, animal welfare considerations play a major role. The aim is to achieve a cage design that allows tests to be carried out under stress-free conditions for the animals; this increases the quality of the results as far as their meaningfulness is concerned. To emphasize our previous experience, TSE Systems was the sole industrial partner in the EU PHENOSCALE™ project that also covers the collection of metabolic data from mice.

Key personnel

Dr. Silvia Brenda, chemist and technical writer, RATstream™ project leader (January 2007 - June 2010), a European project on the "Characterization of transgenic rat models for neurodegenerative and psychiatric diseases: Automated home cage analyses, live imaging and treatment". Dr. Thomas Budiman, a physicist and division manager of Inhalation and Metabolism, involved in the development of new techniques and products, technical consulting for pre- and after-sales as well as responsible for after-sales service. Dipl.-Ing. Kai Holländer is working for TSE since March 2011 and responsible for development projects as „Project Assistant Development":

Selected references

  • Beiroa D, Romero-Pico A, Langa C, Bernabeu C, Lopez M, et al. 2013. Heterozygous Deficiency of Endoglin Decreases Insulin and Hepatic Triglyceride Levels during High Fat Diet, PLoS ONE 8(1): e54591. PMID: 23336009
  • He C, et al. 2012. Exercise–induced BCL2–regulated autophagy is required for muscle glucose homeostasis, Nature. 2012 January 18; 481(7382): 511–515. PMID: 22258505
  • Benz V, Bloch M, Wardat S, Bo¨hm C, Maurer L, et al. 2012. Sexual Dimorphic Regulation of Body Weight Dynamics and Adipose Tissue Lipolysis, PLoS ONE 7(5): e37794. doi:10.1371/journal.pone.0037794. PMID: 22662224
Universitat Autonoma de Barcelona, Barcelona, Spain

 CBATEG Logo

CBATEG
Edifici H
Campus de la UAB
08193 Bellaterra (Cerdanyola del Vallès)
Spain

 CBATEG Building

The Centre of Animal Biotechnology and Gene Therapy (CBATEG), headed by Prof. Fatima Bosch, is specialized in the study of the pathophysiology of metabolic diseases, especially diabetes mellitus and obesity, and their secondary complications (retinopathy, nephropathy and neuropathy). Research is based on transgenic animal models and aims to develop new gene therapy approaches to these diseases. The CBATEG has established a core of platforms that offer metabolic, in vivo imaging, morphological and pathological mouse phenotypic analyses to internal and external users. CBATEG has recently been upgraded to the CBATEG-Mouse Clinic, with the support of the Catalan and Spanish governments. This will allow for the development of a high-throughput platform for the generation and phenotyping of transgenic mouse models.

Key personnel

Prof. Fatima Bosch, Director of CBATEG, Full Professor of Biochemistry and Molecular Biology, School of Veterinary Medicine, UAB. Member of "Genetic Diseases Committee" of the American Society of Gene and Cell Therapy (ASGT), USA and also of the European Society of Gene and Cell Therapy (ESGT), Vice-President of the European Association for the Study of Diabetes (EASD); Dr. Tura Ferre, Highly specialized technician, expertise in somatic mutagenesis, physiology, metabolism and clinical biochemistry; Dr. Sylvie Franckhauser, Research Associate, expertise in somatic mutagenesis, physiology and metabolism; Dr. Pedro Otaegui, Veterinary Medical Doctor, Research Associate, expertise in mouse mutagenesis, somatic mutagenesis and generation and maintenance of mouse colonies; Prof. Marti Pumarola, Veterinary Medical Doctor, Full Professor of Animal Medicine and Surgery, Expertise in Histopathological and immunohistochemical analysis, microscopical analysis; Dr. Anna Pujol, highly specialized technician, expertise in mouse mutagenesis, generation of transgenic and knock-out mice; Prof. Jesus Ruberte, Veterinary Medical Doctor, Full Professor of Animal Health and Anatomy with expertise in mouse embryology and morphology, histological, microscopical and In vivo Imaging Analysis

Selected references

  • Whole body correction of mucopolysaccharidosis IIIA by intracerebrospinal fluid gene therapy. Haurigot V., Marcó S., Ribera A., Garcia M., Ruzo A., Villacampa P., Ayuso E., Añor S., Andaluz A., Pineda M., García-Fructuoso G., Molas M., Maggioni L., Muñoz S., Motas S., Ruberte J., Mingozzi F. ,Pumarola M., Bosch F. J Clin Invest. 2013; PMID :23863627
  • Insulin-like Growth Factor I (IGF-I)-induced Chronic Gliosis and Retinal Stress Lead to Neurodegeneration in a Mouse Model of Retinopathy.Villacampa P, Ribera A, Motas S, Ramírez L, García M, de la Villa P, Haurigot V, Bosch F.J Biol Chem. 2013 288(24):17631-42. PMID: 23620587
  • Vascular endothelial growth factor-mediated islet hypervascularization and inflammation contribute to progressive reduction of β-cell mass. Agudo J, Ayuso E, Jimenez V, Casellas A, Mallol C, Salavert A, Tafuro S, Obach M, Ruzo A, Moya M, Pujol A, Bosch F. Diabetes. 2012 Nov;61(11):2851-61. PMID: 22961079
  • Adipose tissue overexpression of vascular endothelial growth factor protects against diet-induced obesity and insulin resistance. Elias I, Franckhauser S, Ferré T, Vilà L, Tafuro S, Muñoz S, Roca C, Ramos D, Pujol A, Riu E, Ruberte J, Bosch F. Diabetes. 2012 Jul;61(7):1801-13. PMID: 22522611
  • Phosphofructo-1-kinase deficiency leads to a severe cardiac and hematological disorder in addition to skeletal muscle glycogenosis. García M, Pujol A, Ruzo A, Riu E, Ruberte J, Arbós A, Serafín A, Albella B, Felíu JE, Bosch F. PLoS Genet. 2009 Aug;5(8) PMID:19696889
University of Copenhagen, Copenhagen, Denmark

KU logo

University of Copenhagen
Nørregade 10
DK-1165 Copenhagen
Denmark

UC Building

The University of Copenhagen is the largest institution of research and education in Denmark with more than 38 000 students and over 9000 employees. The Transgenic Mouse Core Facility of the Faculty of Health is a service unit, which helps scientists and companies to generate and archive genetically modified mice. Our aim is to provide an excellent transgenic service addressing the individual needs of each user, compensating the small size by increased flexibility. With respect to archiving, our goal is to establish a Danish EMMA node to let Danish scientists take full advantage of this European Research Infrastructure network.

Key personnel

Prof. Dr. Cord Brakebusch is the Director of the Transgenic Mouse Core Facility and a group leader at the Biomedical Institute and at BRIC, an excellence centre in biomedical research. Cord Brakebusch participates in funded networks on phenotyping and archiving of mice in Denmark and Northern Europe. Dr. Javier Martin is the expert technician of the Transgenic Mouse Core Facility with more than 10 years experience in transgenic services in academia and industry.

Selected references

  • Brakebusch, C., Pihlajaniemi, T. 2011. (eds) "Mouse as a Model Organism-From Animals to Cells", Springer, Dordrecht, The Netherlands.
  • Brakebusch, C. 2011. Generation and analysis of genetically modified mice. I: Handbook of Laboratory Animal Science Volume I: Essential Principles and Practices 3rd edition. Editors: Hau, J., Schapiro, S. J. CRC Press, Taylor & Francis Group. Boca Raton, London, New York.
University of Oulu, Oulu, Finland

UOulu Logo

University of Oulu
P.O. Box 8000,
FI-90014 University of Oulu
Finland

UOulu Building

The University of Oulu, founded in 1958, is an international science university which creates innovation for the future, well-being, and knowledge through multidisciplinary research and education. The research and education community of Oulu University consists of 16 000 students and 3000 employees, and it is one of the biggest and the most multidisciplinary universities in Finland. The six faculties, the many departments and the specialized research units of the University of Oulu create the foundation for multi-scientific research, innovation and training of experts for demanding professional tasks.

The Biocenter Oulu (BCO) is the spear head of research in biosciences and molecular medicine at the University of Oulu. The BCO transgenic core facility has been systematically developed to an open-access, full-service facility, and it provides services of high-quality. New laboratories designed and dedicated for these activities started operation in 2004 and were upgraded in 2010 to serve as the Finnish EMMA node. The Oulu University Experimental Animal Centre has an SPF barrier for 12 000 mice, a recently renovated conventional facility for 12 000 mice, as well as a specific quarantine unit.

Key personnel

Prof. Dr. Taina Pihlajaniemi, MD, acts as  Vice President (Vice Rector) Provost for Science and Research Professor of Medical Biochemistry and is affiliated with Center for Cell-Matrix Research, Biocenter Oulu, and Department of Medical Biochemistry and Molecular Biology. Dr. Raija Soininen is the Coordinator of the BCO transgenic core facility.

Mouse resource enquiries: emma-oulu@oulu.fi

Selected references

  • Laitala A, Aro E, Walkinshaw G, Mäki JM, Rossi M, Heikkilä M, Savolainen E-R, Arend M, Kivirikko KI, Koivunen P, Myllyharju J. 2012. A transmembrane prolyl 4-hydroxylase is a fourth prolyl 4-hydroxylase regulating erythropoietin production and erythropoiesis. Blood120: 3336-3344. PMID: 22955912
  • Veikkolainen V, Naillat F, Railo A, Chi L, Manninen A, Hohenstein P, Hastie N, Vainio S, Elenius K 2012. ErbB4 modulates tubular cell polarity and lumen diameter during kidney development. J Am Soc Nephrol 23, 112-122. PMID: 22076439
  • Aro E, Khatri R, Gerard-O’Riley R, Mangiavini L, Myllyharju J,Schipani E. 2012. Hypoxia-inducible factor-1 (HIF-1) but not HIF-2 is essential for hypoxic induction of collagen prolyl 4-hydroxylases in primary epiphyseal growth plate chondrocytes. J Biol Chem 287: 37134-37144. PMID: 22930750
University of Veterinary Medicine, Vienna, Austria

VUW logo

University of Veterinary Medicine Vienna
Institute of Laboratory Animal Science
A-1210 Vienna
Austria
VUW Campus

The University of Veterinary Medicine Vienna (Veterinärmedizinische Universität Wien, VUW) is the only academic, educational, and research institution for veterinary medicine in Austria. In addition to applied and clinical research, VUW conducts excellent basic research in veterinary medicine in the areas of animal health, comparative medicine, animal models and public health services.
The Vienna EMMA node is physically and organisationally localised at the Institute of Laboratory Animal Science (ILAS) and the Biomodels Austria platform (BIAT); part of the department for biomedical sciences. VUW-ILAS and BIAT address the increasing demands of animal experimentation for interdisciplinary research in the life sciences such as genetic engineering, experimental standards, hygienic quality, biosafety, and animal welfare. With this unique expertise, VUW-ILAS is involved in numerous research projects locally, nationally, and worldwide. Furthermore, VUW-ILAS is active in the advanced training of post graduates (FELASA courses), the academic education of students of veterinary medicine and biomedicine / biotechnology, and in the public understanding of the importance of biosciences to society. Newly equipped laboratories for the generation of transgenic rodents by a variety of techniques and for the application of methods of assisted reproduction are available. We routinely perform SPF rederivations of contaminated strains for removal of pathogens to improve the hygienic quality of laboratory rodents before experimental use. Moreover, VUW-BIAT runs the only public archive in Austria for the cryopreservation of mouse mutants.

Key personnel

Prof. Dr. Thomas Rülicke, full professor for Laboratory Animal Science, and head of the Institute of Laboratory Animal Science; board member and participating director of the European Mouse Mutant Archive (EMMA) has experience in breeding and genetics of laboratory animals, transgenesis in mammalian biomodels, genotype and phenotype characterization of mouse mutants, methods of assisted reproduction in laboratory rodents. Dr. Auke Boersma, EMMA archive manager; trained as veterinarian and specialized in veterinary reproduction, long-standing experience in mouse sperm and embryo cryopreservation, involved in the EMMA project since 2001. Olga Olszanska is an advanced technician and manages all laboratory activities related to the animal facility. She is actively involved in the generation of transgenic mice, rederivation of contaminated strains, cryopreservation and revitalization of archived mouse models.

Mouse resource enquiries: emma-vienna@vetmeduni.ac.at

Selected references

  • Pichlmair, A; Lassnig, C; Eberle, CA; Górna, MW; Baumann, CL; Burkard, TR; Bürckstümmer, T; Stefanovic, A; Krieger, S; Bennett, KL; Rülicke, T; Weber, F; Colinge, J; Müller, M; Superti-Furga, G 2011. IFIT1 is an antiviral protein that recognizes 5'-triphosphate RNA. Nat Immunol 12: 624-630. PMID: 21642987
  • Nindl, V; Maier, R; Ratering, D; De Giuli, R; Züst, R; Thiel, V; Scandella, E; Di Padova, F; Kopf, M; Rudin, M; Rülicke, T; Ludewig, B 2012. Cooperation of Th1 and Th17 cells determines transition from autoimmune myocarditis to dilated cardiomyopathy. Eur J Immunol 42: 2311-2321.
    PMID: 22730043
  • Wallner, B; Leitner, NR; Vielnascher, RM; Kernbauer, E; Kolbe, T; Karaghiosoff, M; Rülicke, T; Decker, T; Müller, M. 2012. Generation of mice with a conditional Stat1 null allele. Transgenic Res 21: 217-224.
    PMID: 21553074